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1 H, 13 C, 15 N backbone resonance assignment of the recognition lobe subdomain 3 (Rec3) from Streptococcus pyogenes CRISPR-Cas9
Biomolecular NMR Assignments ( IF 0.9 ) Pub Date : 2020-09-15 , DOI: 10.1007/s12104-020-09977-0
Erin Skeens 1 , Kyle W East 1 , George P Lisi 1
Affiliation  

Rec3 is a subdomain of the recognition (Rec) lobe within CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-associated protein Cas9 that is involved in nucleic acid binding and is critical to HNH endonuclease activation. Here, we report the backbone resonance assignments of an engineered construct of the Rec3 subdomain from Streptococcus pyogenes Cas9. We also analyze backbone chemical shift data to predict secondary structure and an overall fold that is consistent with that of Rec3 from the full-length S. pyogenes Cas9 protein.



中文翻译:

1 H, 13 C, 15 N 来自化脓性链球菌 CRISPR-Cas9 的识别叶子域 3 (Rec3) 的骨架共振分配

Rec3 是 CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)相关蛋白 Cas9 中识别 (Rec) 瓣的一个子域,参与核酸结合并对 HNH 核酸内切酶激活至关重要。在这里,我们报告了来自Streptococcus pyogenes Cas9的 Rec3 子域的工程构建体的骨架共振分配。我们还分析了骨架化学位移数据以预测二级结构和与全长化脓性链球菌Cas9 蛋白的 Rec3 一致的整体折叠。

更新日期:2020-09-15
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