Natural cannabinoids may have beneficial effects on various tissues and functions including a positive influence on the immune system and the inflammatory process. The purpose of this study was to investigate the effects of natural cannabinoids on the production of pro-inflammatory cytokines by lipopolysaccharide (LPS)-stimulated whole human blood cells. Levels of the pro-inflammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were measured before and after exposure of LPS-stimulated whole blood to different concentrations of Cannabidiol (CBD) or a combination of CBD and Tetrahydrocannabinol (THC) extract. LPS stimulated the production of the pro-inflammatory cytokines. Exposure to both CBD and CBD/THC extracts significantly suppressed cytokine production in a dose-dependent manner. Exposure to cannabinoid concentrations of 50 µg/ml or 100 µg/ml resulted in a near-complete inhibition of cytokine production. This study demonstrates that natural cannabinoids significantly suppress pro-inflammatory cytokine production in LPS-stimulated whole blood in a dose-dependent manner. The use of human whole blood, rather than isolated specific cells or tissues, may closely mimic an in vivo sepsis environment. These findings highlight the role that natural cannabinoids may play in suppressing inflammation and call for additional studies of their use as possible novel therapeutic agents for acute and chronic inflammation.

天然大麻素可能对各种组织和功能产生有益作用，包括对免疫系统和炎症过程产生积极影响。这项研究的目的是研究天然大麻素对脂多糖（LPS）刺激的全人类血细胞促炎细胞因子产生的影响。在LPS刺激的全血暴露于不同的前后，测量促炎细胞因子白细胞介素1β（IL-1β），白细胞介素6（IL-6）和肿瘤坏死因子-α（TNF-α）的水平。浓度的大麻二酚（CBD）或CBD与四氢大麻酚（THC）提取物的组合。LPS刺激了促炎细胞因子的产生。暴露于CBD和CBD / THC提取物均以剂量依赖性方式显着抑制细胞因子的产生。暴露于50 µg / ml或100 µg / ml的大麻素浓度几乎完全抑制了细胞因子的产生。这项研究表明，天然大麻素以剂量依赖的方式显着抑制LPS刺激的全血中促炎性细胞因子的产生。使用人类全血而不是分离的特定细胞或组织可以紧密模拟体内脓毒症环境。这些发现突出了天然大麻素在抑制炎症中的作用，并呼吁对其作为急性和慢性炎症的新型治疗剂的用途进行进一步的研究。这项研究表明，天然大麻素以剂量依赖的方式显着抑制LPS刺激的全血中促炎性细胞因子的产生。使用人类全血而不是分离的特定细胞或组织可以紧密模拟体内脓毒症环境。这些发现突出了天然大麻素在抑制炎症中的作用，并呼吁对其作为急性和慢性炎症的新型治疗剂的用途进行进一步的研究。这项研究表明，天然大麻素以剂量依赖的方式显着抑制LPS刺激的全血中促炎性细胞因子的产生。使用人类全血而不是分离的特定细胞或组织可以紧密模拟体内脓毒症环境。这些发现突出了天然大麻素在抑制炎症中的作用，并呼吁对其作为急性和慢性炎症的新型治疗剂的用途进行进一步的研究。