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Activities of Succinate Dehydrogenase and Lactate Dehydrogenase in Blood Lymphocytes in Yakut Ground Squirrels Spermophilus undulatus During Hibernation and in the Active State
Bulletin of Experimental Biology and Medicine ( IF 0.7 ) Pub Date : 2020-08-01 , DOI: 10.1007/s10517-020-04906-6
N V Khunderyakova 1 , N M Zakharova 2
Affiliation  

We studied energy metabolism in blood lymphocytes of Yakut ground squirrels Spermophilus undulatus in active state and during hibernation. Activities of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), marker enzymes of mitochondrial respiration and glycolysis, were measured by a cytobiochemical method based on quantitative assessment of a product of NBT reduction to diformazan in blood lymphocytes immobilized on glass. To measure SDH and LDH activities, cytobiochemical staining of immobilized cells was performed with succinate, lactate, and NAD. In the state of hibernation, SDH activity decreased by 3 times and LDH activity decreased by 10 times or more. These results suggest that the decrease in metabolic activity in lymphocytes of ground squirrels during hypothermia is associated with inhibition of glycolysis, rather than with mitochondrial energy supply.

中文翻译:

雅库特地松鼠在冬眠和活跃状态下血淋巴细胞中琥珀酸脱氢酶和乳酸脱氢酶的活性

我们研究了活跃状态和冬眠期间雅库特地松鼠 Spermophilus undulatus 血液淋巴细胞的能量代谢。琥珀酸脱氢酶 (SDH) 和乳酸脱氢酶 (LDH) 的活性是线粒体呼吸和糖酵解的标记酶,通过细胞生化方法测量,该方法基于固定在玻璃上的血液淋巴细胞中 NBT 还原为二甲脒的产物的定量评估。为了测量 SDH 和 LDH 活性,用琥珀酸盐、乳酸盐和 NAD 对固定细胞进行细胞生化染色。在冬眠状态下,SDH活性下降3倍,LDH活性下降10倍以上。这些结果表明,低温期间地松鼠淋巴细胞代谢活性的降低与糖酵解的抑制有关,
更新日期:2020-08-01
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