We studied spatial organization and structural characteristics of striatal glial cells in spontaneously hypertensive rats (SHR) in 48 h after 30-min focal ischemia. Immunocytochemical analysis of nestin and glial fibrillar acidic protein (GFAP) revealed 3 types of activated astrocytes: expressing only nestin, only GFAP, or both markers. There were no nestin-immunopositive astrocytes in the striatum of sham-operated rats. In cells expressing nestin and GFAP, localization of these markers did not completely coincide, which can be explained by different functions of these proteins or formation of heterodimers of nestin with other intermediate filament proteins.

中文翻译：

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巢蛋白和胶质纤维酸性蛋白在 SHR 大鼠脑边缘缺血区的表达

我们研究了局部缺血 30 分钟后 48 小时内自发性高血压大鼠 (SHR) 纹状体神经胶质细胞的空间组织和结构特征。巢蛋白和胶质纤维酸性蛋白 (GFAP) 的免疫细胞化学分析揭示了 3 种类型的活化星形胶质细胞：仅表达巢蛋白、仅表达 GFAP 或两种标记物。假手术大鼠的纹状体中没有巢蛋白免疫阳性的星形胶质细胞。在表达巢蛋白和 GFAP 的细胞中，这些标记的定位并不完全一致，这可以通过这些蛋白质的不同功能或巢蛋白与其他中间丝蛋白的异二聚体形成来解释。