5-Aminolevulinic acid hydrochloride (ALA)-mediated sonodynamic therapy (SDT) had anti-tumour effect on pancreatic cancer cells. Hence, ALA loaded lipid/poly(lactic-co-glycolic acid) (PLGA) microbubbles (MBs)-mediated SDT for pancreatic cancer has great potential. The average size of ALA-lipid MBs and ALA-PLGA MBs was about 3.0 µm. The two kinds of MBs had good biocompatibility to normal HPDE6-C7 cells and were not toxic to pancreatic cancer cells. Compared with ALA-induced SDT, a statistically significant decrease in cell viability was observed in ALA lipid/PLGA MBs combined with ultrasound groups in AsPC-1 and BxPC-3 cells (p < .05). Obvious effect on the apoptotic rate, apoptosis and pyroptosis morphology, enhanced reactive oxygen species was found in ALA-lipid/PLGA MBs mediated SDT in vitro. Through in vivo study, we found ALA-lipid/PLGA MBs-mediated SDT was a promise treatment for pancreatic cancer.

中文翻译：

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载有5-氨基乙酰丙酸盐酸盐的微泡介导的胰腺癌细胞声动力学治疗。

5-氨基乙酰丙酸盐酸盐（ALA）介导的声动力学疗法（SDT）对胰腺癌细胞具有抗肿瘤作用。因此，载有ALA的脂质/聚乳酸-乙醇酸（PLGA）微泡（MBs）介导的SDT对胰腺癌具有巨大的潜力。ALA-脂质MB和ALA-PLGA MB的平均尺寸为约3.0μm。两种MB与正常的HPDE6-C7细胞具有良好的生物相容性，对胰腺癌细胞无毒性。与ALA诱导的SDT相比，在ALPC脂质/ PLGA MBs与超声组结合的AsPC-1和BxPC-3细胞中观察到了细胞活力的统计学显着下降（p <.05）。在体外ALA-脂质/ PLGA MBs介导的SDT中，对细胞凋亡率，凋亡和发烧形态有明显影响，并增加了活性氧。通过体内研究