Objectives To elucidate the role of FSH1 (family of serine hydrolase) in lipid homeostasis. Results Proteins in various species containing alpha/beta hydrolase domain are known to be involved in lipid metabolism. In silico analysis of the FSH1 gene in Saccharomyces cerevisiae revealed the presence of alpha/beta hydrolase domain (ABHD) and a lipase motif (GXSXG), however its function in lipid metabolism remained elusive. The overexpression of FSH1 in WT and fsh1Δ cells showed a significant reduction in the cellular phospholipid levels and an increase in the triacylglycerol levels and lipid droplet (LD) number. Furthermore, the purified recombinant protein Fsh1p was identified as a lysophospholipase that specifically acts on lysophosphatidylserine (LPS) and impacts the lipid homeostasis in S. cerevisiae . Conclusions These results depicted that Fsh1p has a role on lipid homeostasis and is a lysophospholipase that hydrolyzes lysophosphatidylserine (LPS).

中文翻译：

---

FSH1 在酿酒酵母中编码溶血磷脂酶活性

目的阐明FSH1（丝氨酸水解酶家族）在脂质稳态中的作用。结果已知包含α/β水解酶结构域的各种物种中的蛋白质参与脂质代谢。对酿酒酵母中 FSH1 基因的计算机分析揭示了 α/β 水解酶域 (ABHD) 和脂肪酶基序 (GXSXG) 的存在，但其在脂质代谢中的功能仍然难以捉摸。FSH1 在 WT 和 fsh1Δ 细胞中的过表达显示细胞磷脂水平显着降低，甘油三酯水平和脂滴 (LD) 数量增加。此外，纯化的重组蛋白 Fsh1p 被鉴定为一种溶血磷脂酶，它专门作用于溶血磷脂酰丝氨酸 (LPS) 并影响酿酒酵母中的脂质稳态。