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Phylogenetic Classification, Biofilm-Forming Capacity, Virulence Factors, and Antimicrobial Resistance in Uropathogenic Escherichia coli (UPEC)
Current Microbiology ( IF 2.6 ) Pub Date : 2020-09-10 , DOI: 10.1007/s00284-020-02173-2
Rosa Baldiris-Avila 1 , Alfredo Montes-Robledo 1, 2 , Yaleyvis Buelvas-Montes 3
Affiliation  

Uropathogenic Escherichia coli (UPEC) is the main cause of urinary tract infections; in recent years, its importance as a pathogen has increased due to the emergence of hypervirulent and multiresistant strains. In this study, 190 urinary isolates of E. coli were assigned into the seven phylogenetic groups A (11.1%), B1 (4.7%), B2 (46.8%), C (5.8%) D (25.3%) F (2.6%), and Clade I (2.1%), and various virulence genes were examined with polymerase chain reaction methods. All isolates had at least one virulence factor of the 9 analyzed fyuA (81.1%), fimH (96.8%), iutA (74.7%), ompT (66.8%), kpsMTII (66.8%), traT (58.9%), PAI (43.6%), PapAH (26.3%), and usp (3.2%). The results showed a direct relationship between the virulence factors and phylogenetic group A and B2. Further, virulence genetic profiles fimH, fyuA, ompT, traT, and kpsMTII correlated with the production of strong biofilm, multidrug resistance, and the production of moderate hemolysin. These results suggest that these strains may become reservoirs of genes that encode virulence factors, which could be transferred horizontally enhancing their genomic background and high possibility of acquiring new genetic information for possible dissemination. This study provides the first description of phylogroups in UPEC in the Colombian Caribbean and the association with virulence factor profile, antimicrobial susceptibility, and their possible role in the epidemiology in Colombia.

中文翻译:

泌尿致病性大肠杆菌 (UPEC) 的系统发育分类、生物膜形成能力、毒力因子和抗菌素耐药性

尿路致病性大肠杆菌 (UPEC) 是尿路感染的主要原因;近年来,由于高毒力和多重耐药菌株的出现,其作为病原体的重要性有所增加。在这项研究中,190 株尿液大肠杆菌被分配到七个系统发育组 A (11.1%)、B1 (4.7%)、B2 (46.8%)、C (5.8%) D (25.3%) F (2.6%) ) 和 Clade I (2.1%),并用聚合酶链反应方法检查了各种毒力基因。所有分离株在 9 个分析的 fyuA (81.1%)、fimH (96.8%)、iutA (74.7%)、ompT (66.8%)、kpsMTII (66.8%)、traT (58.9%)、PAI ( 43.6%)、PapAH (26.3%) 和 USP (3.2%)。结果表明毒力因子与系统发育组A和B2之间存在直接关系。此外,毒力遗传谱 fimH、fyuA、ompT、traT、kpsMTII 与强生物膜的产生、多药耐药性和中度溶血素的产生相关。这些结果表明,这些菌株可能成为编码毒力因子的基因库,这些基因可以水平转移,增强它们的基因组背景,并且很有可能获得新的遗传信息以进行传播。本研究首次描述了哥伦比亚加勒比地区 UPEC 的系统群,以及与毒力因子谱、抗菌药物敏感性的关联,以及它们在哥伦比亚流行病学中的可能作用。可以横向转移,增强他们的基因组背景和获取新遗传信息以进行传播的可能性很高。本研究首次描述了哥伦比亚加勒比地区 UPEC 的系统群,以及与毒力因子谱、抗菌药物敏感性的关联,以及它们在哥伦比亚流行病学中的可能作用。可以横向转移,增强他们的基因组背景和获取新遗传信息以进行传播的可能性很高。本研究首次描述了哥伦比亚加勒比地区 UPEC 的系统群,以及与毒力因子谱、抗菌药物敏感性的关联,以及它们在哥伦比亚流行病学中的可能作用。
更新日期:2020-09-10
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