Nickel boride is generally used in the steel industry as a melting accelerator due to its feature of creating a protective and stable attribute at high temperatures. It is also used to improve the hardenability of the steel with boron addition in the production. Thus, safety studies and biocompatibility analysis of nickel boride should be performed comprehensively to understand the limitations of use in various areas. In the present study, nickel boride nanoparticles (Ni₂B NPs) were synthesized by a single-step method and molecule characterizations were performed via the use of X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) analyses. Cytotoxicity properties of Ni₂B NPs were identified on human pulmonary alveolar epithelial cells (HPAEpiC) by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), neutral red (NR), and lactate dehydrogenase (LDH) assays. Illumina human ht-12 v4.0 whole-genome microarray analysis was conducted to investigate NiB₂ NPs effects on gene expression regulations of HPAEpiC cells. The database for annotation, visualization, and integrated discovery (DAVID) analysis was performed to reveal the relationship between Ni₂B NP application and cellular pathway alterations. According to cytotoxicity analysis, the IC₅₀ value for Ni₂B NP application was found as 81.99 mg/L concentration. Microarray analysis of Ni₂B NP application was shown for the first time that 693 gene expression changes (FC ≥ 2) occurred significantly over 40.000 gene probes and Ni₂B NPs were observed to affect microtubule regulation, centrosome organization, and phosphoprotein synthesis.

硼化镍通常在钢铁工业中用作熔化促进剂，因为它具有在高温下产生保护性和稳定特性的特性。也用于生产中加硼提高钢的淬透性。因此，应全面进行硼化镍的安全性研究和生物相容性分析，以了解在各个领域使用的局限性。在本研究中，硼化镍纳米粒子 (Ni ₂ B NPs) 通过一步法合成，并通过使用 X 射线衍射分析 (XRD)、透射电子显微镜 (TEM)、扫描电子显微镜进行分子表征(SEM) 和能量色散 X 射线 (EDX) 分析。Ni _{2 的}细胞毒性特性通过使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑 (MTT)、中性红 (NR) 和乳酸脱氢酶 (LDH) 在人肺泡上皮细胞 (HPAEpiC) 上鉴定出 B NPs ) 化验。进行了 Illumina 人类 ht-12 v4.0 全基因组微阵列分析以研究 NiB ₂ NPs 对 HPAEpiC 细胞基因表达调控的影响。进行注释、可视化和集成发现 (DAVID) 分析的数据库以揭示 Ni ₂ B NP 应用与细胞通路改变之间的关系。根据细胞毒性分析，Ni ₂ B NP 应用的IC ₅₀值为81.99 mg/L 浓度。Ni _{2 的}微阵列分析B NP 应用首次显示，超过 40.000 个基因探针显着发生了 693 个基因表达变化（FC ≥ 2），并且观察到Ni ₂ B NP 影响微管调节、中心体组织和磷蛋白合成。