Anesthesia is used to sedate aquatic animals during transportation or to immobilize them for surgery. However, most studies have focused on the behavioral effects of induction and recovery, without addressing the effect of anesthetic on neural activity. This study investigated the neural response of anterior lateral line afferent fibers in the oyster toadfish, Opsanus tau, during exposure to incremental increases of AQUI-S 20E (0.001-0.006%), to determine if eugenol (the active ingredient of AQUI-S 20E) influences neural activity of the fish lateral line system. Ventilation rate significantly decreased following AQUI-S 20E exposure with the surgical plane of anesthesia reached at 0.003%, characterized by shallow ventilation, equilibrium loss, and no response to tactile stimuli. Spontaneous and evoked firing rates of anterior lateral line fibers also significantly decreased following exposure, although the effect was transitory as neural activity recovered in the majority of fibers (70%) within 30 min of anesthetic withdrawal. While AQUI-S 20E proved effective in inducing the surgical plane of anesthesia without compromising survival, it is not recommended for acute neural preparations due to its depression of neural activity. However, the depression of lateral line sensitivity at low concentrations could play a role in reducing the stress response during fish transport.

中文翻译：

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AQUI-S20E®（丁香酚）对牡蛎蟾蜍（Opsanus tau）传入神经活性的麻醉作用。

麻醉用于在运输过程中养活水生动物或将其固定以进行手术。但是，大多数研究都集中在诱导和恢复的行为效应上，而没有涉及麻醉药对神经活动的影响。这项研究调查了牡蛎蟾鱼Opsanus tau暴露于AQUI-S 20E（0.001-0.006％）增量增加期间前外侧入路纤维的神经反应，以确定丁香酚（AQUI-S 20E的活性成分） ）影响鱼侧线系统的神经活动。AQUI-S 20E暴露后，手术麻醉面通气率为0.003％，通气率显着降低，其特征在于通气浅，平衡丧失，并且对触觉刺激无反应。暴露后，前外侧线纤维的自发和诱发放电速率也显着降低，尽管这种作用是短暂的，因为在麻醉退出后30分钟内，大多数纤维（70％）中的神经活动恢复了。虽然AQUI-S 20E被证明可以有效地诱导麻醉的手术平面而不损害其生存率，但由于其神经活性降低，因此不建议用于急性神经制剂。但是，低浓度下侧线敏感性的降低可能在减少鱼类运输过程中的应激反应中起作用。虽然AQUI-S 20E被证明可以有效地诱导麻醉的手术平面而不损害其生存率，但由于其神经活性降低，因此不建议用于急性神经制剂。但是，低浓度下侧线敏感性的降低可能在减少鱼类运输过程中的应激反应中起作用。虽然AQUI-S 20E被证明可以有效地诱导麻醉的手术平面而不损害其生存率，但由于其神经活性降低，因此不建议用于急性神经制剂。但是，低浓度下侧线敏感性的降低可能在减少鱼类运输过程中的应激反应中起作用。