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Effect of Exclusive Enteral Nutrition on Th17 Cells in Juvenile Rats with Inflammatory Bowel Disease.
Inflammation ( IF 5.1 ) Pub Date : 2020-09-08 , DOI: 10.1007/s10753-020-01328-4
Xu Teng 1 , Yingying Qi 1 , Jing Li 1 , Jie Wu 1, 2
Affiliation  

The objective was to investigate the effect of exclusive enteral nutrition (EEN) on T helper (Th) 17 cells by observing the effects of EEN on colon and serum interleukin (IL)-17A levels in juvenile inflammatory bowel disease (IBD) rat models and to reveal the potential mechanism of the therapeutic effect of EEN on IBD. ATNBS-induced IBD rat model was established. Feeding Peptison, a type of enteric nutrition (EN) for EEN-IBD group and EEN group, normal feed for IBD model group and control group for six consecutive days. Four groups of juvenile rats were sacrificed on day 7. The pathology of the intestinal mucosa was examined, the expression of IL-17A in serum was detected by ELISA, and the expression of IL-17A in intestinal tissue was detected by both western blot and real-time PCR (RT-PCR). Diarrhea, bloody stools, and weight loss were found in both the IBD group and the EEN-IBD group. After 5 days of EEN feeding, the stool characteristics, and blood in the stools of the rats in the EEN-IBD group were significantly relieved compared with those of the IBD group. There was no significant difference in the body mass growth rate between the IBD group and EEN-IBD group (P > 0.05). The growth rate of the EEN group was 51.29 ± 3.61%, which was significantly lower than that of the control group (60.17 ± 9.32%) with P < 0.05. The disease activity index (DAI) score of the EEN-IBD group was significantly lower than that of the IBD group (P < 0.05). In the IBD group, colonic congestion and edema were obvious, scattered ulcers were observed, and the intestinal mucosa had a large amount of inflammatory cell infiltration. In the EEN-IBD group, the intestinal mucosa was slightly congested and a small amount of inflammatory cell infiltrated. The serum IL-17A expression level in the IBD group was significantly higher than in the EEN-IBD group, control group, and EEN group (P < 0.05). Both the gene and protein expressions of IL-17A in the intestinal tissue of the EEN-IBD group were significantly lower than in the IBD group (P < 0.01), and it was significantly higher in the IBD group than in the control and EEN groups (P < 0.01). EEN effectively reduced the intestinal inflammation in the juvenile rats with IBD. The mechanism could be related to the regulation of Th17 cells and the expression of the corresponding cytokine, IL-17A. EEN may play a role in downregulating the expression of IL-17A in the intestinal mucosa.



中文翻译:

纯肠内营养对幼年炎症性肠病大鼠Th17细胞的影响。

目的是通过观察 EEN 对幼年炎症性肠病 (IBD) 大鼠模型中结肠和血清白细胞介素 (IL)-17A 水平的影响,研究纯肠内营养 (EEN) 对 T 辅助 (Th) 17 细胞的影响。揭示EEN对IBD治疗作用的潜在机制。建立ATNBS诱导的IBD大鼠模型。EEN-IBD组和EEN组喂食Peptison,一种肠内营养(EN),IBD模型组和对照组连续6天正常喂养。第7天处死4组幼鼠,观察肠黏膜病理,ELISA检测血清IL-17A的表达,Western blot和免疫印迹法检测肠组织IL-17A的表达。实时荧光定量 PCR (RT-PCR)。腹泻、便血、IBD 组和 EEN-IBD 组均发现体重减轻。饲喂EEN 5 d后,EEN-IBD组大鼠的粪便性状、便血较IBD组明显减轻。IBD组与EEN-IBD组的体重增长率无显着差异(P > 0.05)。EEN组生长率为51.29±3.61%,显着低于对照组(60.17±9.32%),P <0.05。EEN-IBD组疾病活动指数(DAI)评分显着低于IBD组(P < 0.05)。IBD组结肠充血水肿明显,可见散在溃疡,肠黏膜有大量炎性细胞浸润。EEN-IBD组肠黏膜轻度充血,少量炎性细胞浸润。IBD组血清IL-17A表达水平显着高于EEN-IBD组、对照组和EEN组(P< 0.05)。EEN-IBD组肠组织IL-17A基因和蛋白表达均显着低于IBD组(P < 0.01),IBD组显着高于对照组和EEN组( P < 0.01)。EEN 有效减少了 IBD 幼鼠的肠道炎症。其机制可能与 Th17 细胞的调节和相应细胞因子 IL-17A 的表达有关。EEN 可能在下调肠黏膜 IL-17A 表达中发挥作用。

更新日期:2020-09-08
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