AIM To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infection and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH)2 ], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and RvD2 and in periapical tissues. METHODOLOGY Thirty-six single-rooted teeth with primary endodontic infections and apical periodontitis were selected and randomly divided into three groups according to the medication: [Ca(OH)2 ]+ saline solution (SSL) [Ca(OH)2 + SSL group] (n=12), Ca(OH)2 + 2% chlorhexidine gel [Ca(OH)2 + 2% CHX gel group] (n=12), and NAC [NAC group] (n=12). Samples were collected from the periapical interstitial fluid at two different sampling times: before (S1) and after 14 days of intracanal medications (S2). Resolvins were measured using the enzyme-linked immunosorbent assay. Data were analysed using paired t-test, Wilcoxon test, and Kruskal-Wallis test, followed by Dunn's post hoc test, all statistical tests were performed at a significance level of 5%. RESULTS RvE1 and RvD2 were detected in 100% of the samples (36/36) at S1 and S2. Ca(OH)2 medication did not increase the levels of RvE1 or RvD2 (both p>0.05); however, NAC significantly increased the levels of RvE1 and RvD2 after 14 days of treatment (p<0.05). CONCLUSIONS RvE1 and RvD2 were detected in periapical tissues from teeth with root canal infections. Moreover, calcium hydroxide medication did not increase the levels of resolvins in apical periodontitis. In contrast, the use of NAC intracanal medication significantly increased the levels of RvE1 and RvD2 after 14 days of treatment.

中文翻译：

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氢氧化钙和N-乙酰半胱氨酸对根尖性牙周炎中Resolvins E1和D2水平的临床影响。

目的研究原发性牙髓感染和根尖周炎的牙齿中Resolvins E1（RvE1）和D2（RvD2）的存在，并评估氢氧化钙药物[Ca（OH）2]与2％洗必泰凝胶的结合作用（2％CHX凝胶）和RvE1和RvD2以及根尖周组织中的N-乙酰半胱氨酸（NAC）。方法选择了36颗原发性牙髓感染和根尖性牙周炎的单根牙齿，并根据药物随机分为三组：[Ca（OH）2] +盐水溶液（SSL）[Ca（OH）2 + SSL组]（n = 12），Ca（OH）2 + 2％洗必太凝胶[Ca（OH）2 + 2％CHX凝胶组]（n = 12）和NAC [NAC组]（n = 12）。在两个不同的采样时间从根尖周组织液中收集样品：在导管内药物治疗之前（S1）和14天之后（S2）。使用酶联免疫吸附测定法测量分辨素。数据分析采用配对t检验，Wilcoxon检验和Kruskal-Wallis检验，然后进行Dunn事后检验，所有统计检验的显着性水平为5％。结果在S1和S2的100％的样品（36/36）中检测到RvE1和RvD2。Ca（OH）2药物未增加RvE1或RvD2的水平（均p> 0.05）；然而，NAC在治疗14天后显着增加了RvE1和RvD2的水平（p <0.05）。结论在根管感染的牙齿根尖周组织中检测到RvE1和RvD2。此外，氢氧化钙药物并没有增加根尖性牙周炎中分辨素的水平。相反，在治疗14天后，使用NAC管内药物显着增加了RvE1和RvD2的水平。