Mycogenic synthesis of medically applied zinc oxide (ZnO) and copper oxide (CuO) nanoparticles (NPs) were exploited using Penicillium chrysogenum. The biogenesis and capping processes of the produced nano-metals were conducted by functional fungal extracellular enzymes and proteins. The obtained ZnO-NPs and CuO-NPs were characterized. Also, the antibacterial activity and minimum inhibitory concentration (MIC) values of ZnO-NPs and CuO-NPs were determined. Also, antibiofilm and antifungal activities were investigated. Results have demonstrated the ability of the bio-secreted proteins to cape and reduce ZnO and CuO to hexagonal and spherical ZnO-NPs and CuO-NPs with particle size at 9.0–35.0 nm and 10.5–59.7 nm, respectively. Both ZnO-NPs and CuO-NPs showed high antimicrobial activities not only against Gram-positive and Gram-negative bacteria but also against some phytopathogenic fungal strains. Besides this, those NPs showed varied antibiofilm effects against different microorganisms. Quantitative and qualitative analyses indicated that CuO-NPs had an effective antibiofilm activity against Staphylococcus aureus and therefore can be applied in diverse medical devices. Thus, the mycogenic green synthesized ZnO-NPs and CuO-NPs have the potential as smart nano-materials to be used in the medical field to limit the spread of some pathogenic microbes.

使用Penicillium chrysogenum开发了医用氧化锌（ZnO）和氧化铜（CuO）纳米颗粒（NPs）的真菌合成. 产生的纳米金属的生物发生和加帽过程是通过功能性真菌细胞外酶和蛋白质进行的。对获得的 ZnO-NPs 和 CuO-NPs 进行表征。此外，还测定了 ZnO-NPs 和 CuO-NPs 的抗菌活性和最小抑菌浓度 (MIC) 值。此外，还研究了抗生物膜和抗真菌活性。结果表明，生物分泌蛋白能够将 ZnO 和 CuO 剥离和还原成六边形和球形的 ZnO-NPs 和 CuO-NPs，粒径分别为 9.0-35.0 nm 和 10.5-59.7 nm。ZnO-NPs 和 CuO-NPs 均显示出高抗菌活性，不仅对革兰氏阳性和革兰氏阴性细菌，而且对一些植物致病真菌菌株也表现出很高的抗菌活性。除此之外，这些纳米颗粒对不同微生物表现出不同的抗生物膜作用。金黄色葡萄球菌，因此可应用于各种医疗器械。因此，真菌绿色合成的 ZnO-NPs 和 CuO-NPs 有可能作为智能纳米材料用于医学领域，以限制一些病原微生物的传播。