In this study, a quantitative 1H NMR method (1H-qNMR) for determining the contents of imperatorin, byakangelicin, and oxypeucedanin in A. dahurica in traditional Chinese medicine (TCM) has been established. Dried plant material was extracted exhaustively with methanol by an ultrasonication-assisted extraction method. The 1H-qNMR measurements were performed on a 600 -MHz spectrometer with hydroquinone as the internal standard reference in deuterated dimethyl sulfoxide (DMSO-d6) solvent. Quantification was carried out using the 1H resonance signals at 6.55 ppm for hydroquinone and 7.68, 7.38-7.39, and 6.38-6.39 ppm for imperatorin, byakangelicin, and oxypeucedanin, respectively. The linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, reproducibility, stability, and recovery of the methodology were evaluated, and results were good. The newly developed method has been applied to determine the three coumarins in A. dahurica.

中文翻译：

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1H-qNMR法同时测定当归中的三种香豆素：一种快速有效的原料药质量控制方法

在这项研究中，建立了一种定量的1H NMR方法（1H-qNMR），用于测定中草药（TCM）中水飞蓟宾中欧前胡素，拜康力汀和氧平丹的含量。通过超声辅助提取方法用甲醇彻底提取干燥的植物材料。1H-qNMR测量在氘化二甲基亚砜（DMSO-d6）溶剂中以对苯二酚为内标参照物的600 MHz光谱仪上进行。使用1H共振信号对氢醌进行定量，对欧前胡素，Byakangelicin和oxypeucedanin分别为6.55 ppm和7.68、7.38-7.39和6.38-6.39 ppm。评估了方法的线性，检测限（LOD），定量限（LOQ），精密度，重现性，稳定性和回收率，结果很好。新开发的方法已被用于测定A草中的三种香豆素。