当前位置: X-MOL 学术Int. J. Genom. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Comparative Transcriptome Analysis Reveals Molecular Defensive Mechanism of Arachis hypogaea in Response to Salt Stress
International Journal of Genomics ( IF 2.9 ) Pub Date : 2020-02-27 , DOI: 10.1155/2020/6524093
Hao Zhang 1, 2 , Xiaobo Zhao 2 , Quanxi Sun 2 , Caixia Yan 2 , Juan Wang 2 , Cuiling Yuan 2 , Chunjuan Li 2 , Shihua Shan 2 , Fengzhen Liu 1
Affiliation  

Abiotic stresses comprise all nonliving factors, such as soil salinity, drought, extreme temperatures, and metal toxicity, posing a serious threat to agriculture and affecting the plant production around the world. Peanut (Arachis hypogaea L.) is one of the most important crops for vegetable oil, proteins, minerals, and vitamins in the world. Therefore, it is of importance to understand the molecular mechanism of peanut against salt stress. Six transcriptome sequencing libraries including 24-hour salt treatments and control samples were constructed from the young leaves of peanut. A comprehensive analysis between two groups detected 3,425 differentially expressed genes (DEGs) including 2,013 upregulated genes and 1,412 downregulated genes. Of these DEGs, 141 transcription factors (TFs) mainly consisting of MYB, AP2/ERF, WRKY, bHLH, and HSF were identified in response to salinity stress. Further, GO categories of the DEGs highly related to regulation of cell growth, cell periphery, sustained external encapsulating structure, cell wall organization or biogenesis, antioxidant activity, and peroxidase activity were significantly enriched for upregulated DEGs. The function of downregulated DEGs was mainly enriched in regulation of metabolic processes, oxidoreductase activity, and catalytic activity. Fourteen DEGs with response to salt tolerance were validated by real-time PCR. Taken together, the identification of DEGs’ response to salt tolerance of cultivated peanut will provide a solid foundation for improving salt-tolerant peanut genetic manipulation in the future.

中文翻译:

比较转录组分析揭示了花生对盐胁迫的防御机制。

非生物胁迫包括所有非生物因素,例如土壤盐分,干旱,极端温度和金属毒性,这对农业构成了严重威胁,并影响了全世界的植物生产。花生(花生)L.)是世界上最重要的植物油,蛋白质,矿物质和维生素作物之一。因此,了解花生抗盐胁迫的分子机制具有重要意义。从花生的幼叶中构建了六个转录组测序文库,包括24小时盐处理和对照样品。两组之间的全面分析检测到3,425个差异表达基因(DEG),包括2,013个上调基因和1,412个下调基因。在这些DEG中,响应盐分胁迫,鉴定出主要由MYB,AP2 / ERF,WRKY,bHLH和HSF组成的141个转录因子。此外,DEG的GO类别与细胞生长,细胞外围,持续的外部包囊结构,细胞壁组织或生物发生,抗氧化活性,过氧化物酶活性和过氧化物酶活性显着丰富了上调的DEGs。下调的DEG的功能主要集中在代谢过程,氧化还原酶活性和催化活性的调节上。通过实时PCR验证了十四种对盐耐性有响应的DEG。综上所述,确定DEG对栽培花生耐盐性的响应将为将来改善耐盐花生基因操作提供坚实的基础。
更新日期:2020-02-27
down
wechat
bug