当前位置: X-MOL 学术Web Ecol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Salt tolerance's toll: prolonged exposure to saline water inflicts damage to the blood cells of dice snakes (Natrix tessellata)
Web Ecology ( IF 2.2 ) Pub Date : 2017-01-24 , DOI: 10.5194/we-17-1-2017
Vanya Koleva , Yurii Kornilev , Ivan Telenchev , Simeon Lukanov , Berna Hristova , Nikolay Natchev

Abstract. Dice snakes (Natrix tessellata, Laurenti, 1768) inhabit oligohaline and brackish waters along the Bulgarian Black Sea coast, where they often forage at sea. Under these conditions, this species should tolerate highly variable blood plasma sodium concentrations for extended time periods, but the effect of high sodium concentrations to blood cell morphology and physiology is largely unknown. In this experiment, we placed adult dice snakes in waters with different salinity for 84 h, representing the three typical saline concentrations in which dice snakes live and forage: 0.2 ‰ NaCl, fresh water, 16 ‰ NaCl, Black Sea water and 36 ‰ NaCl, World Ocean water. We collected blood through cardiocentesis after exposure to each treatment and used Giemsa's solution for staining the formed elements. We registered numerous nuclear abnormalities (nuclear buds, lobes and blebs) in the blood cells of all investigated specimens. In the snakes placed in the waters with high saline concentration (16 and 36 ‰ NaCl) we found numerous cells with ruptured membranes. These snakes also showed increased mitochondrial activity compared to those in fresh water (1.85 and 2.53 times higher, respectively). Our data suggest that even though dice snakes show a remarkable tolerance to waters with increased salinity, prolonged exposure to it induces significant physiological stress in N. tessellata, which poses a clear limit for their ability to stay in the sea for a long time.

中文翻译:

耐盐性的代价:长时间暴露在盐水中会对骰子蛇(<i>Natrix tessellata</i>)的血细胞造成损害

摘要。骰子蛇 (Natrix tessellata, Laurenti, 1768) 栖息在保加利亚黑海沿岸的贫盐和微咸水域,它们经常在海上觅食。在这些条件下,该物种应在较长时间内耐受高度可变的血浆钠浓度,但高钠浓度对血细胞形态和生理学的影响在很大程度上是未知的。在本实验中,我们将成年骰子蛇置于不同盐度的水域中84 h,代表了骰子蛇生活和觅食的三种典型盐水浓度:0.2‰ NaCl、淡水、16‰ NaCl、黑海水和36‰ NaCl ,世界海洋水。我们在每次治疗后通过心脏穿刺术收集血液,并使用吉姆萨溶液对有形成分进行染色。我们在所有研究样本的血细胞中登记了许多核异常(核芽、裂片和气泡)。在放置在高盐浓度(16 和 36 ‰ NaCl)的水中的蛇中,我们发现了许多膜破裂的细胞。与淡水中的蛇相比,这些蛇的线粒体活性也有所增加(分别高出 1.85 和 2.53 倍)。我们的数据表明,尽管骰子蛇对盐度增加的水域表现出显着的耐受性,但长时间接触它会导致 N. tessellata 产生显着的生理压力,这对其在海中长时间停留的能力构成了明显限制。在放置在高盐浓度(16 和 36 ‰ NaCl)的水中的蛇中,我们发现了许多膜破裂的细胞。与淡水中的蛇相比,这些蛇的线粒体活性也有所增加(分别高出 1.85 和 2.53 倍)。我们的数据表明,尽管骰子蛇对盐度增加的水域表现出显着的耐受性,但长时间接触它会导致 N. tessellata 产生显着的生理压力,这对其在海中长时间停留的能力构成了明显限制。在放置在高盐浓度(16 和 36 ‰ NaCl)的水中的蛇中,我们发现了许多膜破裂的细胞。与淡水中的蛇相比,这些蛇的线粒体活性也有所增加(分别高出 1.85 和 2.53 倍)。我们的数据表明,尽管骰子蛇对盐度增加的水域表现出显着的耐受性,但长时间接触它会导致 N. tessellata 产生显着的生理压力,这对其在海中长时间停留的能力构成了明显限制。
更新日期:2017-01-24
down
wechat
bug