Background: MBT, a rubber accelerator, was popularly used in rubber manufacturing. However, the experts from the International Agency for Research on Cancer suggested that MBT was listed in Group 2A carcinogenic material.

Methods: Therefore, we developed a quantification method based on LC-QqQ-MS/MS, using isotopic iodoacetamide and 13C2, D2-iodoacetamide to react with the thiol group on MBT to generate the iodoacetamide-modified MBT as a standard for a calibration curve and the 13C2, D2-iodoacetamidemodified MBT analog as an internal standard.

Results: Using LC-QqQ-MS/MS, we explored a Multiple Reaction Monitoring (MRM) MS detection mode by detecting m/z of precursor and product ions of MBT, and this method was validated using linear range, LOD, LOQ, intra-day, inter-day, and average recoveries. This validated method was successfully applied to a waste tire as a real sample.

Conclusion: By a complete synthesis with isotopic iodoacetamide alkylation, MBT could be modified with iodoacetamide and 13C2, D2-iodoacetamide with high yields. Furthermore, in MS detection, the signal enhancement could be observed clearly after alkylation. Therefore, this modification of MBT with isotopic iodoacetamide vastly improved the detection of MBT by mass spectrometry.

背景：MBT，一种橡胶促进剂，广泛用于橡胶制造中。但是，国际癌症研究机构的专家建议将MBT列入2A组致癌物质。

方法：因此，我们开发了一种基于LC-QqQ-MS / MS的定量方法，该方法使用同位素碘乙酰胺和13C2，D2-碘乙酰胺与MBT上的巯基反应生成碘乙酰胺修饰的MBT作为校正曲线的标准以及13C2，D2-碘乙酰胺修饰的MBT类似物作为内标。

结果：使用LC-QqQ-MS / MS，我们通过检测MBT的前体离子和产物离子的m / z探索了多反应监测（MRM）MS检测模式，并使用线性范围，LOD，LOQ，内标法对该方法进行了验证。日，日间和平均回收率。这种经过验证的方法已成功地应用于废轮胎作为真实样品。

结论：通过同位素碘乙酰胺烷基化的完全合成，可以用碘乙酰胺和13C2，D2-碘乙酰胺对MBT进行高产率的修饰。此外，在MS检测中，烷基化后可以清楚地观察到信号增强。因此，用同位素碘乙酰胺对MBT的这种修饰极大地改善了质谱检测MBT的能力。