Novel polyheterocyclic nitrogen systems such as 2-amino-1H-pyrazolo[5',1':3,4][1,2,4]- triazino[5,6-b]indole-3-carbonitrile 2, which has been synthesized for the first time in the present work, is considered as starting material for the synthesis of more novel polyheterocyclic systems with five fused rings and sometimes eight fused rings via heterocyclization with α,β-bifunctional reagents under different conditions. The formed structures of the products were established from their correct elemental analysis and spectral data. Some of the compounds were evaluated for their antimicrobial activity using the Agar Well Diffusion method. Compounds 2, 4, 5, 6, 8 were found to be biologically active (Supplementary information).

新型多杂环氮系统，例如2-氨基-1H-吡唑并[5'，1'：3,4] [1,2,4]-三嗪基[5,6-b]吲哚-3-腈2在本工作中首次合成的多杂环系统被认为是通过在不同条件下与α，β-双功能试剂杂环化来合成具有五个稠环甚至有时八个稠环的更新颖的多杂环系统的原料。产品的形成结构是根据其正确的元素分析和光谱数据确定的。使用琼脂井扩散法评估了其中一些化合物的抗菌活性。发现化合物2、4、5、6、8具有生物活性（补充信息）。