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Pharmacokinetic Differences of Grape Seed Procyanidins According to the Gavage Administration Between Normal Rats and Alzheimer's Disease Rats
Current Pharmaceutical Analysis ( IF 0.6 ) Pub Date : 2020-12-31 , DOI: 10.2174/1573412915666190916161225
Xinhui Cheng 1 , Jingying Zhang 1 , Huiting Jing 1 , Yu Qi 1 , Tingxu Yan 2 , Bo Wu 2 , Yiyang Du 2 , Feng Xiao 2 , Ying Jia 2
Affiliation  

Background: Grape Seed Procyanidins (GSP) refers to a type of natural polyphenols that have to roust antioxidant capacity. Studies have shed light on the fact that GSP significantly impacts the alleviation of Alzheimer's Disease (AD).

Objective: This study aimed at investigating whether there exists a pharmacokinetics difference in GSP between normal and AD rats, a rapid UPLC-MS/MS methodology, for the detection of its content in plasma samples was put forward. We carried out an analysis of the plasma concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in normal and AD rats over time for determining the plasma concentration of GSP.

Methods: We made use of 400 μL of methanol for the protein precipitation solvent in the plasma treatment. The chromatographic separation was carried out on a C18 column at a temperature of 20 °C. The mobile phase was a gradient of 0.1% formic acid in water and methanol within 15 min.

Results: In the current research work, the plasma concentrations of procyanidin B2, procyanidin B3, catechin and epicatechin in AD rats were significantly higher as compared with those in normal rats (P < 0.05) and the content of epicatechin constituted the highest as compared with catechin, procyanidin B2 and procyanidin B3 following the administration of GSP.

Conclusion: We discovered the better absorptions of these analytes in the AD group as compared with that in the normal group, providing an analytical basis for treating the AD with procyanidins.



中文翻译:

正常大鼠和阿尔茨海默氏病大鼠管饲方式下葡萄籽原花青素的药代动力学差异

背景:葡萄籽原花青素(GSP)是指必须增强抗氧化能力的一种天然多酚。研究表明,普惠制对减轻阿尔茨海默氏病(AD)有重要影响。

目的:本研究旨在研究正常和AD大鼠之间GSP是否存在药代动力学差异,提出了一种快速UPLC-MS / MS方法检测血浆中GSP的含量。我们对正常和AD大鼠随时间的血浆原花青素B2,原花青素B3,儿茶素和表儿茶素的血浆浓度进行了分析,以确定GSP的血浆浓度。

方法:在血浆处理中,我们使用400μL甲醇作为蛋白质沉淀溶剂。在20℃的温度下在C18柱上进行色谱分离。流动相为15分钟内在水和甲醇中的0.1%甲酸梯度。

结果:在目前的研究工作中,AD大鼠血浆中原花青素B2,原花青素B3,儿茶素和表儿茶素的浓度明显高于正常大鼠(P <0.05),表儿茶素的含量最高。给予GSP后,儿茶素,原花青素B2和原花青素B3升高。

结论:我们发现与正常组相比,AD组中这些分析物的吸收更好,这为用原花青素治疗AD提供了分析依据。

更新日期:2020-11-23
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