The leaves of Stauntonia hexaphylla (SHL) are a very popular herbal medicine in Korea because it can be used to treat rheumatic osteoporosis and other diseases. However, owing to the inconsistency in harvesting time and growth years, their quality is uneven, which indirectly negatively affects the safety of this medication. Therefore, the difference of constituents in SHL harvested at different seasons and years were analyzed by high-performance liquid chromatography coupled to photodiode-array and electrospray ionization mass spectrometry detectors (HPLC–PDA–ESI/MS). A total of 15 components were tentatively characterized in samples of SHL, including 5 compounds reported for the first time in this plant. Moreover, the relative content of these constitutions was simultaneously determined by HPLC coupled with evaporative light scattering detection (ELSD). Hierarchical clustering analysis (HCA) and principal component analysis (PCA) revealed that the quality has a certain extent difference in different harvest times, the best harvest time was 3 years old growing in autumn. The same harvest time was also suggested based on the anti-inflammatory evaluation.

中文翻译：

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通过HPLC-PDA-ESI-MS / MS和ELSD结合多变量统计分析和抗炎活性评估，对不同收获时间的六叶金盏花叶片中的十五种成分进行鉴别和质量评估

在韩国，六木木瓜叶子是一种非常受欢迎的草药，因为它可以用于治疗风湿性骨质疏松症和其他疾病。但是，由于收割时间和生长年限不一致，它们的质量参差不齐，从而间接影响了这种药物的安全性。因此，通过高效液相色谱，光电二极管阵列和电喷雾电离质谱检测器（HPLC-PDA-ESI / MS）分析了在不同季节和年份收获的SHL中各成分的差异。初步对SHL样品中的15种成分进行了表征，其中首次报道了5种化合物。此外，通过HPLC结合蒸发光散射检测（ELSD）同时测定这些组成的相对含量。层次聚类分析（HCA）和主成分分析（PCA）显示，不同收获时间的品质有一定程度的差异，最佳收获时间为秋季生长的3岁。基于抗炎评价，也建议相同的收获时间。