SlyA is an important transcriptional regulator in Salmonella typhimurium (S. typhimurium). Numerous reports have indicated the impact of SlyA on the virulence of S. typhimurium. Less information regarding the role of SlyA in the cell metabolism of S. typhimurium is available. To close this gap, we compared the growth kinetics of an S. typhimurium wild-type strain to a slyA deletion mutant strain. The data suggested that the cell growth of S. typhimurium was impaired when slyA abolished, indicating that SlyA might affect the cell metabolism of S. typhimurium. To determine the role of SlyA in cell metabolism, we analyzed the metabolite profiles of S. typhimurium in the presence or absence of slyA using gas chromatography coupled with tandem mass spectrometry (GC-MS-MS). With the aim of appropriately interpreting the results obtained from metabolomics, a transcriptomic analysis on both the wild-type S. typhimurium and the slyA deletion mutant was performed. The metabolome data indicated that several glycolysis and lipid metabolism-associated pathways, including the turnover of glycerolipid, pyruvate, butanoate, and glycerophospholipid, were affected in the absence of slyA. In addition, the mRNA levels of several genes associated with glycolysis and lipid turnover were downregulated when slyA was deleted, including pagP, fadL, mgtB, iacp, and yciA. Collectively, these evidence suggested that SlyA affects the glycolysis and lipid turnover of S. typhimurium at a transcriptional level. The raw data of metabolomics is available in the MetaboLights database with an access number of MTBLS1858. The raw data of transcriptome is available in the Sequence Read Archive (SRA) database with an access number of PRJNA656165.

中文翻译：

---

slyA对鼠伤寒沙门氏菌细胞代谢的影响：转录组学和代谢组学的联合研究。

SlyA是鼠伤寒沙门氏菌（鼠伤寒沙门氏菌）中重要的转录调节因子。许多报道表明，SlyA对鼠伤寒沙门氏菌的毒性有影响。关于SlyA在鼠伤寒沙门氏菌细胞代谢中的作用的信息很少。为了缩小这个差距，我们比较了鼠伤寒沙门氏菌野生型菌株与slyA缺失突变菌株的生长动力学。数据表明，当slyA被废除时，鼠伤寒沙门氏菌的细胞生长受到损害，这表明SlyA可能影响鼠伤寒沙门氏菌的细胞代谢。。为了确定SlyA在细胞代谢中的作用，我们使用气相色谱-质谱联用（GC-MS-MS）分析了在有或没有slyA的情况下鼠伤寒沙门氏菌的代谢谱。为了适当地解释从代谢组学获得的结果，对野生型鼠伤寒沙门氏菌和slyA缺失突变体均进行了转录组分析。代谢组数据表明，在不存在slyA的情况下，几种糖酵解和脂质代谢相关的途径（包括甘油脂，丙酮酸，丁酸和甘油磷脂的周转）受到影响。。此外，删除slyA时，与糖酵解和脂质代谢相关的几个基因的mRNA水平也被下调，包括pagP，fadL，mgtB，iacp和yciA。总体而言，这些证据表明，SlyA在转录水平上影响鼠伤寒沙门氏菌的糖酵解和脂质代谢。代谢组学的原始数据可在MetaboLights数据库中获得，访问号为MTBLS1858。转录组的原始数据可在序列读取档案（SRA）数据库中获得，访问号为PRJNA656165。