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Bushen-Tiansui Formula Improves Cognitive Functions in an Aβ1–42 Fibril-Infused Rat Model of Alzheimer’s Disease
Neural Plasticity ( IF 3.1 ) Pub Date : 2020-09-24 , DOI: 10.1155/2020/8874885 Chenxia Sheng 1 , Panpan Xu 1 , Xinyi Liu 2 , Weijun Peng 1 , Daxiong Xiang 2, 3 , Shilin Luo 2, 3
Neural Plasticity ( IF 3.1 ) Pub Date : 2020-09-24 , DOI: 10.1155/2020/8874885 Chenxia Sheng 1 , Panpan Xu 1 , Xinyi Liu 2 , Weijun Peng 1 , Daxiong Xiang 2, 3 , Shilin Luo 2, 3
Affiliation
Bushen-Tiansui Formula (BTF) was empirically updated from a classical prescription named Kong-Sheng-Zhen-Zhong pill. It is based on the traditional Chinese medicine theory of the mutual relationship between the brain and the kidney and is intended to treat neurodegenerative diseases. This formulation has been used for several years to treat patients with Alzheimer’s disease- (AD-) like symptoms in our clinical department. However, the medicinal ingredients and the mechanisms by which BTF improves cognition and memory functions have not been characterized. In this study, we used UPLC-MS to generate a chromatographic fingerprinting of BTF and identified five possible active ingredients, including stilbene glycoside; epimedin A1, B, and C; and icariin. We also showed that oral administration of BTF reversed the cognitive defects in an Aβ1–42 fibril-infused rat model of AD, protected synaptic ultrastructure in the CA1 region, and restored the expression of BDNF, synaptotagmin (Syt), and PSD95. These effects likely occurred through the BDNF-activated receptor tyrosine kinase B (TrkB)/Akt/CREB signaling pathway. Furthermore, BTF exhibited no short-term or chronic toxicity in rats. Together, these results provided a scientific support for the clinical use of BTF to improve learning and memory in patients with AD.
中文翻译:
补肾天遂方改善阿尔茨海默病 Aβ1-42 原纤维注入大鼠模型的认知功能
补肾天遂方(BTF)是从名为孔生真中丸的经典方剂经验更新而来的。它基于脑肾相通的中医理论,旨在治疗神经退行性疾病。该制剂已在我们的临床部门用于治疗阿尔茨海默病 (AD-) 样症状患者多年。然而,BTF 改善认知和记忆功能的药用成分和机制尚未得到表征。在本研究中,我们使用 UPLC-MS 生成 BTF 的色谱指纹图谱,并确定了五种可能的活性成分,包括二苯乙烯苷;淫羊藿素 A1、B 和 C;和淫羊藿苷。我们还表明,口服 BTF 逆转了 A注入β 1-42原纤维的 AD 大鼠模型,保护 CA1 区域的突触超微结构,并恢复 BDNF、突触结合蛋白 (Syt) 和 PSD95 的表达。这些影响可能是通过 BDNF 激活的受体酪氨酸激酶 B (TrkB)/Akt/CREB 信号通路发生的。此外,BTF 在大鼠中没有表现出短期或慢性毒性。总之,这些结果为临床使用 BTF 改善 AD 患者的学习和记忆提供了科学支持。
更新日期:2020-09-24
中文翻译:
补肾天遂方改善阿尔茨海默病 Aβ1-42 原纤维注入大鼠模型的认知功能
补肾天遂方(BTF)是从名为孔生真中丸的经典方剂经验更新而来的。它基于脑肾相通的中医理论,旨在治疗神经退行性疾病。该制剂已在我们的临床部门用于治疗阿尔茨海默病 (AD-) 样症状患者多年。然而,BTF 改善认知和记忆功能的药用成分和机制尚未得到表征。在本研究中,我们使用 UPLC-MS 生成 BTF 的色谱指纹图谱,并确定了五种可能的活性成分,包括二苯乙烯苷;淫羊藿素 A1、B 和 C;和淫羊藿苷。我们还表明,口服 BTF 逆转了 A注入β 1-42原纤维的 AD 大鼠模型,保护 CA1 区域的突触超微结构,并恢复 BDNF、突触结合蛋白 (Syt) 和 PSD95 的表达。这些影响可能是通过 BDNF 激活的受体酪氨酸激酶 B (TrkB)/Akt/CREB 信号通路发生的。此外,BTF 在大鼠中没有表现出短期或慢性毒性。总之,这些结果为临床使用 BTF 改善 AD 患者的学习和记忆提供了科学支持。