当前位置: X-MOL 学术Dis. Markers › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Evaluation of Altered Glutamatergic Activity in a Piglet Model of Hypoxic-Ischemic Brain Damage Using 1H-MRS
Disease Markers ( IF 3.464 ) Pub Date : 2020-09-24 , DOI: 10.1155/2020/8850816
Yuxue Dang 1 , Xiaoming Wang 1
Affiliation  

Background and Objective. The excitotoxicity of glutamate (Glu) is a major risk factor for neonatal hypoxic-ischemic brain damage (HIBD). The role of excitatory amino acid transporter 2 (EAAT2) and the α-amino-3-hydroxy-5-methyl-4-isoxazole-proprionic acid receptor (AMPAR) subunit GluR2 in mediating the Glu excitotoxicity has always been the hotspot. This study was aimed at investigating the early changes of glutamate metabolism in the basal ganglia following hypoxia-ischemia (HI) in a neonatal piglet model using 1H-MRS. Methods. Twenty-five newborn piglets were selected and then randomly assigned to the control group () and the model group () subjected to HI. HI was induced by blocking bilateral carotid blood flow under simultaneous inhalation of a 6% oxygen mixture. 1H-MRS data were acquired from the basal ganglia at the following time points after HI: 6, 12, 24, and 72 h. Changes in protein levels of EAAT2 and GluR2 were determined by immunohistochemical analysis. Correlations among metabolite concentrations, metabolite ratios, and the protein levels of EAAT2 and GluR2 were investigated. Results. The Glu level sharply increased after HI, reached a transient low level of depletion that approached the normal level in the control group, and subsequently increased again. Negative correlations were found between concentrations of Glu and EAAT2 protein levels (, ) and between the Glu/creatine (Cr) ratio and EAAT2 protein level (, ). Moreover, changes in GluR2 protein level were significantly and negatively correlated with those in Glu level (the absolute Glu concentration, , ; Glu/Cr, , ). Conclusions. Changes in Glu level measured by 1H-MRS were inversely correlated with those in EAAT2 and GluR2 protein levels following HI, and the results demonstrated that 1H-MRS can reflect the early changes of glutamatergic activity in vivo.

中文翻译:

使用 1H-MRS 评估缺氧缺血性脑损伤仔猪模型中改变的谷氨酸能活性

背景和目的。谷氨酸 (Glu) 的兴奋毒性是新生儿缺氧缺血性脑损伤 (HIBD) 的主要危险因素。兴奋性氨基酸转运蛋白 2 (EAAT2) 和α-氨基-3-羟基-5-甲基-4-异恶唑-丙酸受体 (AMPAR) 亚基 GluR2 在介导 Glu 兴奋性毒性中的作用一直是研究热点。本研究旨在调查使用1 H-MRS 的新生仔猪模型中缺氧缺血 (HI) 后基底神经节谷氨酸代谢的早期变化。方法。选取 25 头新生仔猪,随机分配到对照组()和模型组 ()受到 HI。HI 是通过在同时吸入 6% 氧气混合物的情况下阻断双侧颈动脉血流来诱导的。在 HI 后的以下时间点从基底神经节获取1 H-MRS 数据:6、12、24 和 72 小时。通过免疫组织化学分析确定 EAAT2 和 GluR2 蛋白水平的变化。研究了代谢物浓度、代谢物比率和 EAAT2 和 GluR2 的蛋白质水平之间的相关性。结果。HI 后 Glu 水平急剧上升,达到接近对照组正常水平的短暂低耗竭水平,随后再次上升。发现 Glu 浓度和 EAAT2 蛋白水平呈负相关(, )以及 Glu/肌酸 (Cr) 比率和 EAAT2 蛋白水平之间 (, )。此外,GluR2 蛋白水平的变化与 Glu 水平(绝对 Glu 浓度,, ; 谷氨酸/铬,, )。 结论1 H-MRS测量的Glu水平变化与HI后EAAT2和GluR2蛋白水平的变化呈负相关,结果表明1 H-MRS可以反映体内谷氨酸能活性的早期变化。
更新日期:2020-09-24
down
wechat
bug