Targeted gene delivery is important in biomedical research and applications. In this paper, we synergistically combine non-viral chemical materials, magnetic nanoparticles (MNPs), and a physical technique, low-intensity pulsed ultrasound (LIPUS), to achieve efficient and targeted gene delivery. The MNPs are iron oxide super-paramagnetic nanoparticles, coated with polyethyleneimine (PEI), which makes a high positive surface charge and is favorable for the binding of genetic materials. Due to the paramagnetic properties of the MNPs, the application of an external magnetic field increases transfection efficiency while LIPUS stimulation enhances cell viability and permeability. We found that stimulation at the intensity of 30 mW/cm² for 10 minutes yields optimal results with a minimal adverse effect on the cells. By combining the effect of the external magnetic field and LIPUS, the genetic material (GFP or Cherry Red plasmid) can enter the cells. The flow cytometry results showed that by using just a magnetic field to direct the genetic material, the transfection efficiency on HEK 293 cells that were treated by our MNPs was 56.1%. Coupled with LIPUS stimulation, it increased to 61.5% or 19% higher than the positive control (Lipofectamine 2000). Besides, compared with the positive control, our method showed less toxicity. Cell viability after transfection was 63.61%, which is 19% higher than the standard transfection technique. In conclusion, we designed a new gene-delivery method that is affordable, targeted, shows low-toxicity, yet high transfection efficiency, compared to other conventional approaches.

靶向基因的传递在生物医学研究和应用中很重要。在本文中，我们将非病毒性化学材料，磁性纳米颗粒（MNP）和物理技术，低强度脉冲超声（LIPUS）协同结合，以实现高效且有针对性的基因传递。MNP是涂有聚乙烯亚胺（PEI）的氧化铁超顺磁性纳米颗粒，具有高的正表面电荷，有利于遗传物质的结合。由于MNP的顺磁特性，施加外部磁场可提高转染效率，而LIPUS刺激可增强细胞活力和通透性。我们发现以30 mW / cm ²的强度进行刺激10分钟可产生最佳结果，对细胞的不良影响最小。通过结合外部磁场和LIPUS的作用，遗传物质（GFP或Cherry Red质粒）可以进入细胞。流式细胞仪结果表明，仅使用磁场引导遗传物质，经我们的MNP处理的HEK 293细胞转染效率为56.1％。加上LIPUS刺激，它比阳性对照增加了61.5％或19％（Lipofectamine 2000）。此外，与阳性对照相比，我们的方法显示出较小的毒性。转染后的细胞活力为63.61％，比标准转染技术高19％。总之，我们设计了一种价格合理，针对性强，毒性低，转染效率高的新型基因递送方法，