SummaryThe aim of this study was to evaluate different post-shock temperatures for tetraploid induction in the yellowtail tetra Astyanax altiparanae. Newly fertilized eggs were divided into four groups, three were submitted to heat shock (40°C for 2 min) at 24 min post-fertilization (mpf) and another group remained without shock (control). Groups submitted to temperature shock were further separated at the following temperatures: 22°C, 26°C and 28°C. Survival among embryonic development was counted and at hatching the ploidy was analyzed by flow cytometry. The results showed that the post-shock temperature affects the parameters analyzed and, therefore, must be considered for optimization of the production of tetraploid in A. altiparanae. Those data are innovative and could be used in future studies of basic biology in this species.

中文翻译：

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热休克后的回温影响黄尾四倍体的产生

摘要本研究的目的是评估不同的休克后温度在黄尾四倍体诱导中的作用。山燕. 新受精卵分为四组，三组在受精后 24 分钟（mpf）进行热休克（40°C 2 分钟），另一组保持未休克（对照）。经受温度冲击的组在以下温度下进一步分开：22°C、26°C 和 28°C。计数胚胎发育中的存活率，并在孵化时通过流式细胞术分析倍性。结果表明，冲击后温度会影响分析的参数，因此必须考虑优化四倍体的生产。A. altiparanee. 这些数据具有创新性，可用于该物种基础生物学的未来研究。