Pulmonary fibrosis is a kind of interstitial lung disease with architectural remodeling of tissues and excessive matrix deposition. Apart from messenger RNA (mRNA), microRNA (miRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA) could also play important roles in the regulatory processes of occurrence and progression of pulmonary fibrosis. In the present study, the pulmonary fibrosis model was administered with bleomycin. Whole transcriptome sequencing analysis was applied to investigate the expression profiles of mRNAs, lncRNAs, circRNAs, and miRNAs. After comparing bleomycin-induced pulmonary fibrosis model lung samples and controls, 286 lncRNAs, 192 mRNAs, 605 circRNAs, and 32 miRNAs were found to be differentially expressed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to investigate the potential functions of these differentially expressed (DE) mRNAs and non-coding RNAs (ncRNAs). The terms related to inflammatory response and tumor necrosis factor (TNF) signaling pathway were enriched, implying potential roles in regulatory process. In addition, two co-expression networks were also constructed to understand the internal regulating relationships of these mRNAs and ncRNAs. Our study provides a systematic perspective on the potential functions of these DE mRNAs and ncRNAs during PF process and could help pave the way for effective therapeutics for this devastating and complex disease.

肺纤维化是一种间质性肺疾病，具有组织结构上的重塑和过多的基质沉积。除了信使RNA（mRNA），微小RNA（miRNA），长非编码RNA（lncRNA）和环状RNA（circRNA）可能在肺纤维化发生和发展的调控过程中也起着重要作用。在本研究中，肺纤维化模型与博来霉素一起施用。应用全转录组测序分析来研究mRNA，lncRNA，circRNA和miRNA的表达谱。比较博莱霉素诱导的肺纤维化模型肺样品和对照后，发现286个lncRNA，192个mRNA，605个circRNA和32个miRNA差异表达。进行了基因本体论（GO）和《京都议定书》的基因与基因组百科全书（KEGG）分析，以研究这些差异表达（DE）mRNA和非编码RNA（ncRNA）的潜在功能。与炎症反应和肿瘤坏死因子（TNF）信号通路相关的术语被丰富，暗示在调节过程中的潜在作用。此外，还构建了两个共表达网络以了解这些mRNA和ncRNA的内部调节关系。我们的研究为这些DE mRNA和ncRNA在PF过程中的潜在功能提供了系统的观点，并可能有助于为这种破坏性和复杂疾病的有效疗法铺平道路。与炎症反应和肿瘤坏死因子（TNF）信号通路相关的术语被丰富，暗示在调节过程中的潜在作用。此外，还构建了两个共表达网络以了解这些mRNA和ncRNA的内部调节关系。我们的研究为这些DE mRNA和ncRNA在PF过程中的潜在功能提供了系统的观点，并可能有助于为这种破坏性和复杂疾病的有效疗法铺平道路。与炎症反应和肿瘤坏死因子（TNF）信号通路相关的术语被丰富，暗示在调节过程中的潜在作用。此外，还构建了两个共表达网络以了解这些mRNA和ncRNA的内部调节关系。我们的研究为这些DE mRNA和ncRNA在PF过程中的潜在功能提供了系统的观点，并可能有助于为这种破坏性和复杂疾病的有效疗法铺平道路。