Species identification of unknown biological samples is of fundamental importance for forensic applications, especially in crime detection, poaching, and illegal trade of endangered animals as well as meat fraud. In this study, a novel panel was developed to simultaneously identify 10 different animal species (Gallus domesticus, Anas platyrhynchos domesticus, Ovis aries, Sus scrofa domesticus, Bos taurus, Equus caballus, Columba livia domestica, Rattus norvegicus, Mus musculus, and Canis lupus familiaris) and human beings by amplifying 22 short tandem repeat (STR) loci in a multiplex PCR using a set of five fluorescently labeled dyes. This novel 22-STR panel was validated by optimization of PCR conditions as well as species specificity, sensitivity, reproducibility, precision, DNA mixture, and tissue/organ consistency. The results of developmental validation showed that the 22-STR loci achieved high species specificity among 10 animal species and human beings, and the sensitivity of this panel was 0.09 ng. This 22-STR panel identified different meats in mixed samples, and the minimum detected mixture ratio in the current test was 10% (0.1 ng/1 ng). This sensitive, accurate, and specific 22-STR panel can be used for forensic species identification and the detection of meat fraud and adulteration.

未知生物样本的物种识别对于法医学应用至关重要，特别是在犯罪检测，偷猎和濒危动物的非法贸易以及肉类欺诈中。在这项研究中，开发了一个新颖的面板来同时识别10种不同的动物物种（家蝇， 桔梗， 卵巢， 野猪， 金牛座， 马ca， 哥伦比亚家蝇， 褐家鼠， 小家鼠和 犬天狼疮）和人类（通过使用一组5种荧光标记的染料在多重PCR中扩增22个短串联重复序列（STR）位点）。通过优化PCR条件以及物种特异性，敏感性，可重复性，精密度，DNA混合物和组织/器官的一致性，验证了这种新颖的22-STR面板。发育验证的结果表明，22-STR位点在10种动物和人类中具有很高的种属特异性，该面板的灵敏度为0.09 ng。这个22-STR小组确定了混合样品中的不同肉类，当前测试中检测到的最小混合比例为10％（0.1 ng / 1 ng）。这种灵敏，准确且特有的22-STR面板可用于法医物种识别以及肉类欺诈和掺假检测。