Abstract

This study aims to find the association between MYLK4 gene polymorphism and growth traits at different ages, such as birth, 3rd-month, 6th-month and 12th-month ages in Anatolian black cattle. PCR-RFLP method was used to detect the polymorphism for MYLK4 gene in at G61595A locus. Allele and genotype frequencies were A: 0.34, G: 0.66 and AA: 0.17, AG: 0.34 and GG: 0.49. The chi-square χ² test showed an agreement to Hardy–Weinberg equilibrium (p > .01). GLM and RRM models were used to estimate the association between MYLK4 gene polymorphism and growth traits. The results of the statistical analysis indicated an association between MYLK4 gene polymorphism and growth traits at different stage ages in Anatolian black cattle. Therefore, the G61595A mutation of MYLK4 gene can be used as a genetic marker for the selection of growth and development traits in the cattle breeding programs.

摘要

本研究旨在寻找安纳托利亚黑牛MYLK4基因多态性与不同年龄（如出生、第3月、第6月和第12月龄）生长性状之间的关联。PCR-RFLP方法用于检测MYLK4基因在G61595A位点的多态性。等位基因和基因型频率为 A：0.34、G：0.66 和 AA：0.17、AG：0.34 和 GG：0.49。卡方χ ²检验表明与 Hardy-Weinberg 平衡一致 ( p  > .01)。GLM和RRM模型用于估计MYLK4基因多态性与生长性状之间的关联。统计分析的结果表明MYLK4安纳托利亚黑牛不同阶段年龄的基因多态性和生长性状。因此，MYLK4基因的G61595A突变可作为牛育种计划中生长发育性状选择的遗传标记。