Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference materials are required for the standardization of these techniques. This work, initiated from the ISEV 2017 Biomarker Workshop in Birmingham, UK, and with further discussion during the ISEV 2019 Standardization Workshop in Ghent, Belgium, sets out to elucidate which reference materials are required and which are currently available to standardize commonly used analysis platforms for characterizing EV refractive index, epitope abundance, size and concentration. Due to their predominant use among EV researchers, a particular focus is placed on the optical methods nanoparticle tracking analysis and flow cytometry.

细胞外囊泡（EVs）的准确表征对于探索其诊断和治疗应用至关重要。随着电动汽车研究领域的发展，用来表征它们的技术也随之发展。这些技术的标准化需要参考材料的开发。这项工作始于在英国伯明翰举行的ISEV 2017生物标志物研讨会，并在比利时根特举行的ISEV 2019标准化研讨会期间进行了进一步讨论，旨在阐明哪些参考材料是必需的，哪些参考材料目前可用于标准化常用分析平台。用于表征EV折射率，表位丰度，大小和浓度。由于它们在电动汽车研究人员中占主导地位，