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In vitro Studies of Antitumor Effect, Toxicity/Cytotoxicity and Skin Permeation/Retention of a Green Fluorescence Pyrene‐based Dye for PDT Application
Photochemistry and Photobiology ( IF 3.3 ) Pub Date : 2020-10-11 , DOI: 10.1111/php.13335 Bruna Martins de França 1 , Zeinab Ghasemishahrestani 1 , Gabriel Ferreira Motta de Souza 1 , Renata Nascimento da Silva 1 , Daniela Dias Queiroz 1 , Maria Bernadete Riemma Pierre 2 , Marcos Dias Pereira 1 , Josué Sebastián Bello Forero 1 , Rodrigo José Corrêa 1
Photochemistry and Photobiology ( IF 3.3 ) Pub Date : 2020-10-11 , DOI: 10.1111/php.13335 Bruna Martins de França 1 , Zeinab Ghasemishahrestani 1 , Gabriel Ferreira Motta de Souza 1 , Renata Nascimento da Silva 1 , Daniela Dias Queiroz 1 , Maria Bernadete Riemma Pierre 2 , Marcos Dias Pereira 1 , Josué Sebastián Bello Forero 1 , Rodrigo José Corrêa 1
Affiliation
Photosensitizers (PS) are compounds that can generate reactive oxygen species under irradiation of appropriate light and are widely used in photodynamic therapy (PDT). Currently, topical PDT is an effective treatment for several skin diseases, including bacterial infections, fungal mycoses and psoriasis. In addition, PDT is also used to treat non-melanoma skin cancer and can be a potential tool for melanoma, associated with other treatments. In this work, we evaluated the antitumor photoactivity of a new pyrene-based PS (TPPy) by using the murine melanoma cell line (B16F10). The in vitro permeation/retention tests in porcine ear skin were also performed in order to evaluate the potential application of the PS for topical use in skin cancer. Moreover, to determine the toxicity in vivo, we used the Galleria mellonella as an alternative animal model of study. The results showed that TPPy is a promising PS for application in PDT, with potential antitumor photoactivity (IC50 6.5 μmol L-1 ), absence of toxicity in the G. mellonella model at higher concentration (70.0 mmol L-1 ) and the accumulation tendency in the Epidermis plus Dermis sites (165.20 ± 4.12 ng/cm2 ).
中文翻译:
用于 PDT 应用的绿色荧光芘基染料的抗肿瘤作用、毒性/细胞毒性和皮肤渗透/保留的体外研究
光敏剂(PS)是在适当光线照射下能产生活性氧的化合物,广泛用于光动力疗法(PDT)。目前,局部 PDT 是治疗多种皮肤病的有效方法,包括细菌感染、真菌病和银屑病。此外,PDT 还用于治疗非黑色素瘤皮肤癌,并且可以作为黑色素瘤的潜在工具,与其他治疗方法相关联。在这项工作中,我们通过使用鼠黑色素瘤细胞系 (B16F10) 评估了一种新的基于芘的 PS (TPPy) 的抗肿瘤光活性。还进行了猪耳朵皮肤的体外渗透/保留测试,以评估 PS 在皮肤癌中局部使用的潜在应用。此外,为了确定体内毒性,我们使用大蜡螟作为研究的替代动物模型。结果表明,TPPy 是一种用于 PDT 的有前景的 PS,具有潜在的抗肿瘤光活性(IC50 6.5 μmol L-1 ),在高浓度(70.0 mmol L-1 )下在 G. mellonella 模型中没有毒性和积累趋势在表皮加真皮部位 (165.20 ± 4.12 ng/cm2)。
更新日期:2020-10-11
中文翻译:
用于 PDT 应用的绿色荧光芘基染料的抗肿瘤作用、毒性/细胞毒性和皮肤渗透/保留的体外研究
光敏剂(PS)是在适当光线照射下能产生活性氧的化合物,广泛用于光动力疗法(PDT)。目前,局部 PDT 是治疗多种皮肤病的有效方法,包括细菌感染、真菌病和银屑病。此外,PDT 还用于治疗非黑色素瘤皮肤癌,并且可以作为黑色素瘤的潜在工具,与其他治疗方法相关联。在这项工作中,我们通过使用鼠黑色素瘤细胞系 (B16F10) 评估了一种新的基于芘的 PS (TPPy) 的抗肿瘤光活性。还进行了猪耳朵皮肤的体外渗透/保留测试,以评估 PS 在皮肤癌中局部使用的潜在应用。此外,为了确定体内毒性,我们使用大蜡螟作为研究的替代动物模型。结果表明,TPPy 是一种用于 PDT 的有前景的 PS,具有潜在的抗肿瘤光活性(IC50 6.5 μmol L-1 ),在高浓度(70.0 mmol L-1 )下在 G. mellonella 模型中没有毒性和积累趋势在表皮加真皮部位 (165.20 ± 4.12 ng/cm2)。