Based on biomolecular methods, rapid and selective identification of human pathogenic water organisms becomes an important issue. Legionella spp., are pathogenic water bacteria with worldwide significance. Prevalent detection methods for these microorganisms are time and/or cost intensive. We describe a detection setup and relating DNA assay. A miniaturized real‐time polymerase chain reaction (real‐time PCR) for direct on‐line discrimination of Legionella pneumophila and Legionella spp. was established and integrated into a real‐time PCR‐chip‐system. The PCR‐chip device combines a temperature controlling unit and a fluorescence intensity measurement. It was designed to achieve rapid amplification, using an approach of real‐time fluorescence read out with the intercalating dye EvaGreen® and melting curve analysis, without requiring multiple probes. The presented results exhibit reproducibility and good sensitivity, showing that the setup is suitable for robust, rapid and cost‐efficient detection and monitoring of a variety of Legionella spp.in urban water samples.

中文翻译：

---

基于芯片的双重实时PCR用于监测嗜肺军团菌和军团菌属物种的水质。

基于生物分子方法，人类病原性水生物的快速和选择性鉴定成为重要的问题。军团菌属细菌是具有全球意义的致病性水细菌。这些微生物的普遍检测方法是时间和/或成本密集的。我们描述了检测设置和相关的DNA检测。小型实时聚合酶链反应（实时PCR）可直接在线鉴别嗜 肺军团菌和军团菌 spp。建立并集成到实时PCR芯片系统中。PCR芯片设备结合了温度控制单元和荧光强度测量。它设计为使用嵌入的染料EvaGreen®进行实时荧光读取并进行熔解曲线分析，无需多个探针，即可实现快速扩增。呈现的结果具有可重复性和良好的灵敏度，表明该装置适用于对城市水样中的多种军团菌进行健壮，快速和经济高效的检测和监测。