A 60‐day feeding trial was conducted to illustrate the effect of dietary protein levels on the growth and physio‐metabolic responses of juvenile Litopenaeus vannamei reared in inland saline water (ISW). Six isoenergetic (15 MJ/kg) and isolipidic (60 g/kg) diets with graded level of crude protein viz., 240 (T240), 260 (T260), 280 (T280), 300 (T300), 320 (T320) and 340 (T340) g/kg diet were formulated. Significantly higher (p < .05) weight gain (%), specific growth rate, with lower food conversion ratio were found in T320 and T340 groups. The protein utilizing efficiency and whole‐body protein content were significantly higher (p < .05) in the T320 group. Trypsin activity increased with the increasing dietary CP level but amylase activity decreased with the increasing dietary CP level. Transaminase enzymes, haemolymph protein and haemocyanin were elevated in T320 and T340 groups. The lactate dehydrogenase and malate dehydrogenase activities were significantly higher (p < .05) in the T240 group. Shrimp of T240 group had the lowest (p < .05) glycogen and total haemocyte count with highest (p < .05) haemolymph glucose and antioxidant enzymes activities than the other groups. Based on the results, feeding 320 g CP/kg is found to be optimum for supporting maximum growth and health status of L. vannamei reared in ISW at 8 g/L salinity. The finding of the present study will help in developing a low‐cost feed for L. vannamei reared in ISW.

中文翻译：

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日粮蛋白质含量对内陆盐水养殖白虾对虾凡纳滨对虾生长性能，养分利用，消化酶和生理状态的影响

进行了为期60天的喂养试验，以说明日粮蛋白水平对内陆盐水（ISW）饲养的南美白对虾的生长和生理代谢反应的影响。六种等能量（15 MJ / kg）和等脂（60 g / kg）日粮，粗蛋白的分级水平分别为240（T240），260（T260），280（T280），300（T300），320（T320）配制了340（T340）g / kg的饮食。 在T320和T340组中，发现体重增加（％）（p）显着提高（p <.05），食物转化率更低。蛋白质利用效率和全身蛋白质含量明显更高（p <.05）在T320组中。胰蛋白酶活性随日粮CP水平增加而增加，而淀粉酶活性随日粮CP水平增加而降低。T320和T340组的转氨酶，血淋巴蛋白和血蓝蛋白升高。 在T240组中，乳酸脱氢酶和苹果酸脱氢酶的活性明显更高（p <.05）。T240组的虾具有最低的（p  <0.05）糖原和血细胞总数与最高（p  <.05）血淋巴葡萄糖和抗氧化酶活性比其它基团。根据结果​​，饲喂320 g CP / kg是支持南美白对虾最大生长和健康状况的最佳选择在ISW中以8 g / L的盐度饲养。本研究的发现将有助于开发在ISW饲养的南美白对虾的低成本饲料。