The greater wax moth Galleria mellonella is an increasingly popular and consolidated alternative infection model to assess microbial virulence and the effectiveness of antimicrobial compounds. The lack of G. mellonella suppliers aiming at scientific purposes and a lack of well‐established protocols for raising and testing these animals may impact results and reproducibility between different laboratories. In this review, we discuss the state of the art of rearing the larvae in situ, providing an overview of breeding and testing conditions commonly used and their influence on larval health and experiments results, from setting up the environment, providing the ideal diet, understanding the effects of pretreatments, choosing the best testing conditions, to exploring the most from the results obtained. Meanwhile, we guide the reader through the most practical ways of dealing with G. mellonella to achieve successful experiments.

中文翻译：

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饲养和测试条件的概述，以及为科学目的而在实验室中优化黑麦草育种和使用的指南。

更大的蛾蛾梅洛埃勒菌（Galleria mellonella）是一种越来越流行和巩固的替代感染模型，用于评估微生物毒力和抗菌化合物的有效性。缺乏以科学目的为目标的mel。mellonella供应商，并且缺乏完善的饲养和测试这些动物的方案，可能会影响不同实验室之间的结果和可重复性。在这篇评论中，我们讨论了就地饲养幼体的最新技术，从设置环境，提供理想饮食，了解预处理的效果，选择最佳测试条件到从中探索最多的内容，概述了常用的繁殖和测试条件及其对幼虫健康和实验结果的影响。获得的结果。同时，我们引导读者通过最实际的方法应对梅花气单胞菌，以实现成功的实验。