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Feasibility of fecal microbiota transplantation via oral gavage to safely alter gut microbiome composition in marmosets.
American Journal of Primatology ( IF 2.4 ) Pub Date : 2020-09-24 , DOI: 10.1002/ajp.23196
Corinna N Ross 1, 2, 3 , Kelly R Reveles 4, 5
Affiliation  

Disruption of microbial communities within human hosts has been associated with infection, obesity, cognitive decline, cancer risk and frailty, suggesting that microbiome‐targeted therapies may be an option for improving healthspan and lifespan. The objectives of this study were to determine the feasibility of delivering fecal microbiota transplants (FMTs) to marmosets via oral gavage and to evaluate if alteration of the gut microbiome post‐FMT could be achieved. This was a prospective study of marmosets housed at the Barshop Institute for Longevity and Aging Studies in San Antonio, Texas. Eligible animals included healthy young adult males (age 2–5 years) with no recent medication use. Stool from two donors was combined and administered in 0.5 ml doses to five young recipients once weekly for 3 weeks. Safety outcomes and alterations in the gut microbiome composition via 16S ribosomal RNA sequencing were compared at baseline and monthly up to 6 months post‐FMT. Overall, significant differences in the percent relative abundance was seen in FMT recipients at the phylum and family levels from baseline to 1 month and baseline to 6 months post‐FMT. In permutational multivariate analysis of variance analyses, treatment status (donor vs. recipient) (p = .056) and time course (p = .019) predicted β diversity (p = .056). The FMT recipients did not experience any negative health outcomes over the course of the treatment. FMT via oral gavage was safe to administer to young adult marmosets. The marmoset microbiome may be altered by FMT; however, progressive changes in the microbiome are strongly driven by the host and its baseline microbiome composition.

中文翻译:

通过口服管饲法进行粪便微生物群移植以安全改变狨猴肠道微生物组组成的可行性。

人类宿主内微生物群落的破坏与感染、肥胖、认知能力下降、癌症风险和虚弱有关,这表明微生物组靶向疗法可能是改善健康和寿命的一种选择。本研究的目的是确定通过口腔管饲法将粪便微生物群移植 (FMT) 移植到狨猴的可行性,并评估是否可以实现 FMT 后肠道微生物组的改变。这是一项对居住在德克萨斯州圣安东尼奥巴肖普长寿与衰老研究所的狨猴的前瞻性研究。符合条件的动物包括近期未使用药物的健康年轻成年男性(2-5 岁)。来自两名捐赠者的粪便被合并,并以 0.5 毫升的剂量给予五名年轻受者,每周一次,持续 3 周。通过 16S 核糖体 RNA 测序,在基线和每月至 FMT 后 6 个月比较安全性结果和肠道微生物组组成的变化。总体而言,FMT 接受者在门和家族水平上从基线到 FMT 后 1 个月和基线到 6 个月的相对丰度百分比存在显着差异。在方差分析的置换多变量分析中,治疗状态(捐赠者与接受者)(p  = .056) 和时间进程 ( p  = .019) 预测 β 多样性 ( p  = .056)。FMT 接受者在治疗过程中没有经历任何负面的健康结果。通过口服管饲 FMT 对年轻的成年狨猴是安全的。FMT 可能会改变狨猴微生物组;然而,宿主及其基线微生物组组成强烈驱动微生物组的渐进变化。
更新日期:2020-11-19
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