We screened cell line and plasma-derived exosomes for molecules that localize to mitochondria or that reflect mitochondrial integrity. SH-SY5Y cell-derived exosomes contained humanin, citrate synthase, and fibroblast growth factor 21 protein, and plasma-derived exosomes contained humanin, voltage-dependent anion-selective channel 1, and transcription factor A protein. Nuclear mitochondrial (NUMT) DNA complicated analyses of mitochondrial DNA (mtDNA), which otherwise suggested exosomes contain at most very low amounts of extended mtDNA sequences but likely contain degraded pieces of mtDNA. Cell and plasma-derived exosomes contained several mtDNA-derived mRNA sequences, including those for ND2, CO2, and humanin. These results can guide exosome-focused, mitochondria-pertinent biomarker development.

中文翻译：

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外泌体中线粒体相关成分的检测

我们筛选了细胞系和血浆来源的外泌体中定位于线粒体或反映线粒体完整性的分子。SH-SY5Y细胞来源的外泌体含有humanin、柠檬酸合酶和成纤维细胞生长因子21蛋白，血浆来源的外泌体含有humanin、电压依赖性阴离子选择性通道1和转录因子A蛋白。核线粒体 (NUMT) DNA 复杂的线粒体 DNA (mtDNA) 分析，否则表明外泌体最多包含非常少量的扩展 mtDNA 序列，但可能包含降解的 mtDNA 片段。细胞和血浆来源的外泌体包含几个 mtDNA 来源的 mRNA 序列，包括 ND2、CO2 和humanin 的序列。这些结果可以指导以外泌体为重点的线粒体相关生物标志物的开发。