The overall objective in this study is to determine the effects of Bacillus subtilis and Bacillus licheniformis probiotic supplementation on performance and cecal Salmonella load in broilers challenged with Salmonella enterica serovar enteritidis. A total of 360 one-day-old broiler chicks were randomly distributed to 4 experimental groups in a 2 × 2 factorial setup of treatments: control, control + challenge, probiotics (10 mg/kg feed of B. subtilis strain HU58; HU58 plus 100 mg/kg feed of B. licheniformis SC307; Prepro; Microbiome Labs, Saint Augustine, FL), and probiotics + challenge. Each treatment was replicated in 6 pens (n = 6) with 15 chicks per pen. At 21 d of age, all birds in the challenge groups were inoculated orally with 250 μL of 1 × 10⁹ cfu S. enteritidis. At 42 d of age, birds in the control + challenge group had 180-g decrease, whereas birds in the probiotic + challenge groups had only 40-g decrease in the BW gain, compared with the control group. At 28 d of age, birds in the control + challenge group had 220-g decrease, whereas birds in the probiotic + challenge groups had only 140-g decrease in feed consumption compared with that in the control group. Birds in the probiotic + challenge group had 0.73, 1.59, and 1.32 log decrease in Salmonella cfu/g of cecal contents at 5, 12, and 21 d after Salmonella infection, compared with the challenge group. Birds in the challenge group had higher (P < 0.05) titers of anti-Salmonella IgA in the bile. Birds in the probiotic + challenge group had further increased (P < 0.05) anti-Salmonella bile IgA titers compared with the birds in the challenge group. At 21 d after Salmonella infection, birds in the challenge group had 17.3-μm decrease (P < 0.05) in the villus height compared with the control group, whereas birds in the probiotics + challenge group had a comparable villus height to that in the probiotic group. It can be concluded that B. subtilis and B. Licheniformis probiotic can be a tool in decreasing Salmonella loads in the broiler's gastrointestinal tract, and B. subtilis and B. Licheniformis supplementation can be expected to decrease broiler carcass contamination with Salmonella.

在此项研究的总体目标是确定的影响，枯草芽孢杆菌和地衣芽孢杆菌对性能和盲肠补充益生菌沙门氏菌负荷与挑战肉鸡沙门肠炎。总共360只1天大的雏鸡被随机分配到4个实验组，采用2×2因子处理：对照，对照+攻击，益生菌（枯草芽孢杆菌HU58菌株10 mg / kg饲料； HU58加100 mg / kg地衣芽孢杆菌饲料SC307; Prepro; 微生物组实验室，佛罗里达州圣奥古斯丁）和益生菌+挑战。每种处理以6支（n = 6）重复，每支有15只小鸡。在21日龄时，向挑战组中的所有鸟类口服250μL1×10 ⁹  cfu肠炎链球菌。与对照组相比，在42 d龄时，对照组+挑战组的鸟类体重减少180克，而益生菌+挑战组的鸟类体重增加仅40 g的降低。与对照组相比，对照组+挑战组的鸡在28 d时减少220克，而益生菌+挑战组的鸟的饲料消耗仅减少140克。益生菌+攻击组的鸟类沙门氏菌减少0.73、1.59和1.32对数与攻击组相比，沙门氏菌感染后第5、12和21天的盲肠含量为cfu / g盲肠。攻击组的鸟类胆汁 中抗沙门氏菌IgA的滴度较高（P <0.05）。与挑战组相比，益生菌+挑战组的鸟类的沙门氏菌胆汁IgA滴度进一步提高（P  <0.05）。沙门氏菌感染后第21天， 与对照组相比，挑战组的鸟类绒毛高度降低了17.3μm （P <0.05），而益生菌+挑战组的鸟类绒毛高度与益生菌相当组。可以得出结论枯草芽孢杆菌和地衣芽孢杆菌益生菌可以作为降低肉鸡胃肠道沙门氏菌负荷的工具，而补充枯草芽孢杆菌和地衣芽孢杆菌有望减少沙门氏菌对肉鸡car体的污染。