Thiosulfate is frequently used as an energy source and electron donor in autotrophic denitrification (AD) for removing nitrate from wastewater. However, transforming pathways of S₂O₃²⁻ in this process is unclear. Herein, the aim of this study is to explore possible transforming pathways of sulfur compounds in thiosulfate-based AD process. After measuring the variation of NO₃⁻, NO₂⁻, and various sulfur compounds such as S⁰, SO₄²⁻, S₂O₃²⁻, acid volatile sulfide (AVS), and S²⁻ in the presence and absence of S₂O₃²⁻, the variation process of S₂O₃²⁻ and the contribution of various sulfur compounds were analyzed. The results indicated that S⁰, AVS, and S²⁻ were the intermediate products when S₂O₃²⁻ was applied as an electron donor. All S₂O₃²⁻, S⁰, AVS, and S²⁻ could act as electron donors in the nitrate removal process with the final products of SO₄²⁻. The utilization priority of these four sulfur sources was presumed in the following order: S²⁻ > S₂O₃²⁻ > AVS ≈ S⁰. Furthermore, sulfur transformation and balance in nitrate removal process was also investigated. This suggests the transforming pathways of sulfur compounds in denitrification process. Nitrogen removal and sulfur conversion process are dependent on the presence of microorganisms in the sludge.

硫代硫酸盐在自养反硝化（AD）中通常用作能源和电子供体，用于去除废水中的硝酸盐。但是，在该过程中S ₂ O ₃ ^2-的转化途径尚不清楚。在此，本研究的目的是探索基于硫代硫酸盐的AD过程中硫化合物的可能转化途径。测量NO的变化后₃ ^-，NO ₂ ^- ，和各种硫化合物如S ⁰，SO ₄ ^2-，S ₂ ö ₃ ^2-，酸挥发性硫化物（AVS）和S ^2-在存在和不存在的S ₂ O_分析了3 ^2-，S ₂ O ₃ ^2-的变化过程以及各种含硫化合物的贡献。结果表明，当S ₂ O ₃ ^2-被用作电子给体时，S ⁰，AVS和S ^2-是中间产物。所有的S ₂ O ₃ ^2-，S ⁰，AVS和S ^2-都可以在硝酸盐去除过程中作为最终的SO ₄ ^2-的电子给体。这四个硫源的利用优先级按以下顺序推定：S ²⁻  > S ₂O ₃ ²⁻  > AVS≈S ⁰。此外，还研究了硝酸盐去除过程中硫的转化和平衡。这表明了反硝化过程中硫化合物的转化途径。脱氮和硫转化过程取决于污泥中微生物的存在。