Extracellular vesicles (EVs) are membrane-coated particles secreted by virtually all cell types in response to different stimuli, both in physiological and pathological conditions. Their content generally reflects their biological functions and includes a variety of molecules, such as nucleic acids, proteins and cellular components. The role of EVs as signaling vehicles has been widely demonstrated. In particular, they are actively involved in the pathogenesis of several hematological malignancies (HM), mainly interacting with a number of target cells and inducing functional and epigenetic changes. In this regard, by releasing their cargo, EVs play a pivotal role in the bilateral cross-talk between tumor microenvironment and cancer cells, thus facilitating mechanisms of immune escape and supporting tumor growth and progression. Recent advances in high-throughput technologies have allowed the deep characterization and functional interpretation of EV content. In this review, the current knowledge on the high-throughput technology-based characterization of EV cargo in HM is summarized.

细胞外囊泡（EVs）是几乎所有细胞类型在生理和病理条件下响应不同刺激而分泌的膜包裹颗粒。它们的含量通常反映它们的生物学功能，并且包括多种分子，例如核酸，蛋白质和细胞成分。电动汽车作为信号载体的作用已得到广泛证明。特别是，它们积极参与几种血液系统恶性肿瘤（HM）的发病机理，主要与许多靶细胞相互作用并诱导功能性和表观遗传学改变。在这方面，通过释放货物，电动汽车在肿瘤微环境与癌细胞之间的双向串扰中起着关键作用，从而促进了免疫逃逸的机制并支持了肿瘤的生长和发展。高通量技术的最新进展已使EV内容具有更深的表征和功能解释。在这篇评论中