Abstract Poisonous plants are widely distributed and may have risk of phytotoxicity upon mixing with medicinal plants. Several species of Cestrum genus are poisonous and linked with many serious health issues. In the present study, cross-mixing of a toxic plant, Cestrum diurnum with morphologically resembling medicinal plant, Adhatoda vasica was studied using chemical fingerprinting approach. LC-ESI-MS/MS tool was used to develop the chemical fingerprints of three toxic species of Cestrum, including, C. diurnum, C. nocturnum and C. parqui. Total forty-three compounds were identified using high-resolution LC-ESI-MS/MS data comparison. Chemometric analyses were done to compare the distribution of identified compounds present in these Cestrum species. One of the identified compounds, nornicotine (a toxic compound) was also quantified using LC-IT-MS/MS. Adulteration study was conducted by mixing toxic C. diurnum in A. vasica with various ratios (w/w) and five differentiable compounds were identified to detect the adulteration. The method was able to detect up to the limit of 5% mixing of toxic C. diurnum. Moreover, cytotoxicity of the methanolic extracts of these three species were also studied on normal human PBMC (peripheral blood mononuclear cells) and all found to be toxic, while the C. nocturnum showed the highest level of toxicity with the IC50 12.5 μg/mL.

中文翻译：

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使用 LC-ESI-QTOF-MS/MS 通过化学指纹图谱对草本原料中的有毒 Cestrum 物种 Cestrum diurnum 进行交叉混合研究

摘要 有毒植物分布广泛，与药用植物混合可能有药害风险。几种 Cestrum 属有毒，与许多严重的健康问题有关。在本研究中，使用化学指纹法研究了有毒植物 Cestrum diurnum 与形态类似药用植物 Adhatoda vasica 的交叉混合。LC-ESI-MS/MS 工具用于开发三种有毒 Cestrum 物种的化学指纹，包括 C. diurnum、C. nocturnum 和 C. parqui。使用高分辨率 LC-ESI-MS/MS 数据比较共鉴定了 43 种化合物。进行化学计量分析以比较这些 Cestrum 物种中存在的已识别化合物的分布。确定的化合物之一，还使用 LC-IT-MS/MS 对去甲烟碱（一种有毒化合物）进行定量。掺假研究是通过在 A. vasica 中以不同比例 (w/w) 混合有毒的 C. diurnum 进行的，并确定了五种可区分的化合物来检测掺假。该方法能够检测到混合量高达 5% 的有毒 C. diurnum。此外，还研究了这三种物种的甲醇提取物对正常人 PBMC（外周血单核细胞）的细胞毒性，发现它们都具有毒性，而 C. nocturnum 显示出最高水平的毒性，IC50 为 12.5 μg/mL。