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Resistance of a Halobacterium salinarum isolate from a solar saltern to cadmium, lead, nickel, zinc, and copper
Antonie van Leeuwenhoek ( IF 2.6 ) Pub Date : 2020-09-24 , DOI: 10.1007/s10482-020-01475-6
Houda Baati 1 , Mariem Siala 2 , Chafai Azri 1 , Emna Ammar 1 , Christopher Dunlap 3 , Mohamed Trigui 1
Affiliation  

The current study focuses on the tolerance of a strain of Halobacterium salinarum isolated from Sfax solar saltern (Tunisia) towards cadmium (Cd), lead (Pb), nickel (Ni), zinc (Zn), and copper (Cu) by using agar dilution methods in complex and minimal media. The results showed the least inhibitory metals based on Minimum Inhibitory Concentrations (MICs) were lead (MIC = 4.5 mM), cadmium (MIC = 4 mM), and nickel (MIC = 2.5 mM) in complex medium. The MICs of these metals were more inhibitory (MIC < 2 mM) in the other tested media. The archaeal strain revealed a high sensitivity for copper and zinc, with MICs below 0.5 mM for both metals. Growth kinetics in complex and minimal media showed the strain to be more sensitive to the metals in liquid media than in solid media. The growth kinetic assays indicated the presence of selected heavy metals resulted in a lower growth rate and lower total cell mass relative to the control. Despite that cadmium and lead are nonessential and have no nutrient value, they were the most tolerated metals by H. salinarum strain. In addition, pigment intensity in the strain was inhibited by the presence of the heavy metals relative to the control.



中文翻译:

从太阳能盐罐分离出的盐沼嗜盐杆菌对镉,铅,镍,锌和铜的抗性

目前的研究集中在盐沼盐杆菌菌株的耐受性上通过使用琼脂稀释法在复杂且极少的培养基中从Sfax太阳盐厂(突尼斯)分离出镉(Cd),铅(Pb),镍(Ni),锌(Zn)和铜(Cu)。结果表明,在复杂介质中,基于最小抑制浓度(MIC)抑制最小的金属是铅(MIC = 4.5 mM),镉(MIC = 4 mM)和镍(MIC = 2.5 mM)。这些金属的MIC在其他测试介质中更具抑制性(MIC <2 mM)。古细菌菌株显示出对铜和锌的高敏感性,两种金属的MIC均低于0.5 mM。在复杂和基本培养基中的生长动力学表明,该菌株对液体培养基中的金属比对固体培养基更敏感。生长动力学测定表明,与对照相比,所选重金属的存在导致较低的生长速率和较低的总细胞量。H. salinarum菌株。另外,相对于对照,重金属的存在抑制了菌株中的色素强度。

更新日期:2020-09-24
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