Abstract

A comparative analysis of mesenchymal stem cells (MSCs) of differing origin is important because of their specific interaction with a unique microenvironment (niche) in a particular tissue. Some cellular processes are regulated through the interaction of extracellular matrix (ECM) proteins with matrix metalloproteinases (MMPs). In this work, we compared the dynamics of MMP activity and the level of ECM proteins during replicative senescence of three lines of human MSCs obtained from Wharton’s jelly of human umbilical cord (MSCWJ-1 line), eyelid skin (DF-2 line), and human epicardial adipose tissue isolated during coronary artery bypass grafting (ADH-MSC line). The fractions of cells with β-galactosidase enzyme activity (marker of replicative senescence) and the content of ECM proteins (fibronectin and type I collagen), as well as the activity of MMP-1, MMP-2, and MMP-9, were analyzed during long-term cultivation. It was found that three lines differ in the content of fibronectin, type 1 collagen, and MMP activity during the replicative senescence. Cells of the ADH-MSC line are mostly different from the other two lines in terms of aging rate, ECM protein content, and MMP activity. The reason for this discrepancy is that the cells are obtained from the tissue of a patient having heart disease.

中文翻译：

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人间充质干细胞复制衰老过程中基质金属蛋白酶活性和细胞外基质蛋白含量的动态

摘要

不同来源的间充质干细胞（MSC）的比较分析非常重要，因为它们与特定组织中独特的微环境（niche）有特异性相互作用。一些细胞过程通过细胞外基质（ECM）蛋白质与基质金属蛋白酶（MMP）的相互作用来调节。在这项工作中，我们比较了从沃顿氏脐带果冻（MSCWJ-1系），眼睑皮肤（DF-2系）获得的三系人MSC复制衰老过程中MMP活性和ECM蛋白水平的变化，和人心外膜脂肪组织在冠状动脉旁路移植术（ADH-MSC线）中分离。具有β-半乳糖苷酶活性（复制性衰老的标记）和ECM蛋白（纤连蛋白和I型胶原）含量的细胞部分，以及长期培养过程中的MMP-1，MMP-2和MMP-9活性。发现在复制衰老过程中，纤连蛋白，1型胶原和MMP活性的三行不同。ADH-MSC系的细胞在衰老率，ECM蛋白含量和MMP活性方面与其他两个系大不相同。这种差异的原因是细胞是从患有心脏病的患者的组织中获得的。