Genome shuffling, an efficient and practical strain improvement technology via recursive protoplasts fusion, can break through the limits of species even genus to accelerate the directed evolution of microbial strains, without requiring the comprehensively cognized genetic background and operable genetic system. Hence this technology has been widely used for many important strains to obtain the desirable industrial phenotypes. In this review, we introduce the procedure of genome shuffling, discuss the new aid strategies of genome shuffling, summarize the applications of genome shuffling for increasing metabolite yield, improving strain tolerance, enhancing substrate utilization, and put forward the outlook to the future development of this technology.

中文翻译：

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基因组改组在工业微生物菌株改良中的应用及研究进展

基因组改组是一种高效实用的递归原生质体融合菌株改良技术，无需全面认知的遗传背景和可操作的遗传系统，即可突破种属的限制，加速微生物菌株的定向进化。因此，该技术已被广泛用于许多重要菌株以获得理想的工业表型。在这篇综述中，我们介绍了基因组改组的过程，讨论了基因组改组的新辅助策略，总结了基因组改组在提高代谢物产量、提高菌株耐受性、提高底物利用率方面的应用，并对未来的发展提出了展望。这项技术。