White lupin (Lupinus albus L.) is a high-protein grain legume crop, grown since ancient Greece and Rome. Despite long domestication history, its cultivation remains limited, partly because of susceptibility to anthracnose. Only some late-flowering, bitter, low-yielding landraces from Ethiopian mountains displayed resistance to this devastating disease. The resistance is controlled by various genes, thereby complicating the breeding efforts. The objective of this study was developing tools for molecular tracking of Ethiopian resistance genes based on genotyping-by-sequencing (GBS) data, envisaging linkage mapping and genomic selection approaches. Twenty GBS markers from two major quantitative trait loci (QTLs), antr04_1/antr05_1 and antr04_2/antr05_2, were converted to PCR-based markers using assigned transcriptome sequences. Newly developed markers improved mapping resolution around both anthracnose resistance loci, providing more precise QTL estimation. PCR-based screening of diversified domesticated and primitive germplasm revealed the high specificity of two markers for the antr04_1/antr05_1 locus (TP222136 and TP47110) and one for the antr04_2/antr05_2 locus (TP338761), highlighted by simple matching coefficients of 0.96 and 0.89, respectively. Moreover, a genomic selection approach based on GBS data of a recombinant inbred line mapping population was assessed, providing an average predictive ability of 0.56. These tools can be used for preselection of candidate white lupin germplasm for anthracnose resistance assays.

白羽扇豆（羽扇豆albusL.）是一种高蛋白谷物豆类作物，自古希腊和罗马开始种植。尽管驯养历史悠久，但其种植仍然受到限制，部分原因是对炭疽病的敏感性。埃塞俄比亚山区只有一些晚开花，苦，低产的地方品种对这种毁灭性疾病表现出抵抗力。抗性受各种基因控制，从而使育种工作复杂化。这项研究的目的是开发基于序列基因分型（GBS）数据的埃塞俄比亚抗性基因分子追踪工具，设想连锁图谱和基因组选择方法。使用分配的转录组序列，将来自两个主要定量性状基因座（QTL）的20个GBS标记转化为基于PCR的标记，其中两个标记分别是antr04_1 / antr05_1和antr04_2 / antr05_2。新开发的标记物改善了两个炭疽病抗性基因座周围的作图分辨率，从而提供了更精确的QTL估计。基于PCR的多样化驯化和原始种质筛选显示，antr04_1 / antr05_1基因座（TP222136和TP47110）的两个标记和antr04_2 / antr05_2基因座（TP338761）的两个标记具有很高的特异性，突出显示了0.96和0.89的简单匹配系数，分别。此外，评估了基于重组自交系作图群体的GBS数据的基因组选择方法，其平均预测能力为0.56。这些工具可用于炭疽病抗性测定的候选白羽扇豆种质的预选。基于PCR的多样化驯化和原始种质筛选显示，antr04_1 / antr05_1基因座（TP222136和TP47110）的两个标记和antr04_2 / antr05_2基因座（TP338761）的两个标记具有很高的特异性，突出显示了0.96和0.89的简单匹配系数，分别。此外，评估了基于重组自交系作图群体的GBS数据的基因组选择方法，其平均预测能力为0.56。这些工具可用于炭疽病抗性测定的候选白羽扇豆种质的预选。基于PCR的多样化驯化和原始种质筛选显示，antr04_1 / antr05_1基因座（TP222136和TP47110）的两个标记和antr04_2 / antr05_2基因座（TP338761）的两个标记具有很高的特异性，突出显示了0.96和0.89的简单匹配系数，分别。此外，评估了基于重组自交系作图群体的GBS数据的基因组选择方法，其平均预测能力为0.56。这些工具可用于炭疽病抗性测定的候选白羽扇豆种质的预选。评估了基于重组自交系作图群体的GBS数据的基因组选择方法，平均预测能力为0.56。这些工具可用于炭疽病抗性测定的候选白羽扇豆种质的预选。评估了基于重组自交系作图群体的GBS数据的基因组选择方法，平均预测能力为0.56。这些工具可用于炭疽病抗性测定的候选白羽扇豆种质的预选。