We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.

中文翻译：

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CRISPR-Cas12a反切割DNA G-四链体。

我们首次报告激活的CRISPR-Cas12a系统反式切割DNA G-四链体（G4）。通过FRET，CD，凝胶电泳和NMR对人端粒G4和TBA G4的切割活性进行了研究和验证。我们认为，这一发现将为推进CRISPR-Cas12a和G4在生物传感和生物化学中的应用铺平新的途径。