Food consumption during the rest phase promotes circadian desynchrony, which is corrected with harmful physiological and mental disorders. Previously, we found that circadian desynchrony was involved in isoflurane-induced cognitive impairment. Here, we scheduled food access to modulate daily rhythm to examine its impact on isoflurane-induced cognitive impairments. Mice were randomly transferred to restricted feeding (RF) time groups: Control group (Zeitgeber time (ZT) 0–ZT24, ad libitum feeding), Day-Feeding group (ZT0–ZT12, misaligned feeding), and Night-Feeding group (ZT12–ZT24, aligned feeding). Then, some of them were subjected to 5 h of 1.3% isoflurane anaesthesia from ZT14 to ZT19 and were divided into the Control + Anes group, the Day-Feeding + Anes group, and the Night-Feeding + Anes group. Mini-Mitter was used to monitor the daily rhythm. Fear conditioning system was conducted to assess cognition of mice. We observed that the Night-Feeding group adapted to RF gradually, whereas the Day-Feeding group exhibited a disturbed daily rhythm. The Night-Feeding + Anes group exhibited a partially enhanced daily rhythm, whereas the Day-Feeding + Anes group exhibited sustained phase advances and diurnality score increase 7 days after isoflurane anaesthesia. Notably, in tests of hippocampus-dependent contextual memory, the Night-Feeding + Anes group demonstrated decreased deficits; the Day-Feeding + Anes group showed prolonged post-anaesthetic deficits 14 days after isoflurane anaesthesia. However, amygdala-dependent cued-fear conditioning post-anaesthesia was not altered by the RF schedule. In conclusion, we demonstrated that misaligned feeding disturbed the daily rhythm and led to persistent post-anaesthetic cognitive dysfunction. Aligned feeding enhanced the daily rhythm partially and improved post-anaesthetic cognitive dysfunction.

中文翻译：

---

限时进食可改变异氟烷引起的记忆力减退

休息阶段的食物消耗会促进昼夜节律失调，并通过有害的生理和精神障碍来纠正这种情况。以前，我们发现昼夜节律失调与异氟烷引起的认知障碍有关。在这里，我们计划通过食物获取来调节每日节律，以检查其对异氟烷引起的认知障碍的影响。将小鼠随机分为限制性进食（RF）时间组：对照组（Zeitgeber时间（ZT）0–ZT24，随意喂食），白天喂食组（ZT0–ZT12，不对中喂食）和夜间喂食组（ZT12–ZT24，对中喂食）。然后，将其中一些从ZT14到ZT19接受1.3％异氟醚麻醉5小时，分为对照组+麻醉组，白天进食+麻醉组和夜间喂养+麻醉组。使用Mini-Mitter监测每日节律。进行恐惧调节系统以评估小鼠的认知。我们观察到“夜间进食”组逐渐适应了RF，而“白天进食”组表现出紊乱的每日节律。异氟烷麻醉后7天，夜间喂食+ Anes组表现出部分增强的每日节律，而白天喂食+ Anes组表现出持续的相移和昼夜分数增加。值得注意的是，在测试海马依赖的上下文记忆时，Night-Feeding + Anes组的赤字减少；Day-Feeding + Anes组在异氟烷麻醉后14天显示麻醉后虚弱的时间延长。但是，麻醉后杏仁扁桃体依赖的恐惧恐惧状况并没有因RF时间表而改变。总之，我们证明了喂养不当会打乱日常节奏，并导致持续的麻醉后认知功能障碍。对齐喂养部分改善了每日节律，并改善了麻醉后的认知功能障碍。我们证明了喂养不当会打乱日常节奏，并导致持续的麻醉后认知功能障碍。对齐喂养部分改善了每日节律，并改善了麻醉后的认知功能障碍。我们证明了喂养不当会打乱日常节奏，并导致持续的麻醉后认知功能障碍。对齐喂养部分改善了每日节律，并改善了麻醉后的认知功能障碍。