Antarctic regions are characterized by low temperatures and strong UV radiation. This harsh environment is inhabited by psychrophilic and psychrotolerant organisms, which have developed several adaptive features. In this study, we analyzed two Antarctic bacterial strains, Planococcus sp. ANT_H30 and Rhodococcus sp. ANT_H53B. The physiological analysis of these strains revealed their potential to produce various biotechnologically valuable secondary metabolites, including surfactants, siderophores, and orange pigments. The genomic characterization of ANT_H30 and ANT_H53B allowed the identification of genes responsible for the production of carotenoids and the in silico reconstruction of the pigment biosynthesis pathways. The complex manual annotation of the bacterial genomes revealed the metabolic potential to degrade a wide variety of compounds, including xenobiotics and waste materials. Carotenoids produced by these bacteria were analyzed chromatographically, and we proved their activity as scavengers of free radicals. The quantity of crude carotenoid extracts produced at two temperatures using various media was also determined. This was a step toward the optimization of carotenoid production by Antarctic bacteria on a larger scale.

中文翻译：

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基于基因组的南极细菌产生类胡萝卜素的见解，Planococcus sp。ANT_H30 和红球菌属。ANT_H53B

南极地区的特点是低温和强烈的紫外线辐射。这种严酷的环境中栖息着嗜冷和耐寒的生物，它们已经发展出多种适应性特征。在这项研究中，我们分析了两种南极细菌菌株，Planococcus sp。ANT_H30 和红球菌属。ANT_H53B。这些菌株的生理分析揭示了它们产生各种具有生物技术价值的次级代谢物的潜力，包括表面活性剂、铁载体和橙色色素。ANT_H30 和 ANT_H53B 的基因组特征允许鉴定负责产生类胡萝卜素和色素生物合成途径的计算机重建的基因。细菌基因组的复杂手动注释揭示了降解多种化合物的代谢潜力，包括异生物质和废料。对这些细菌产生的类胡萝卜素进行色谱分析，我们证明了它们作为自由基清除剂的活性。还测定了使用不同介质在两种温度下产生的粗类胡萝卜素提取物的量。这是朝着更大规模优化南极细菌生产类胡萝卜素的方向迈出的一步。