Background: The Paulownia Clone in Vitro 112, known as oxytree or oxygen tree, is a hybrid clone of the species Paulownia elongata and Paulownia fortunei (Paulowniaceae). The oxytree is a fast-growing hybrid cultivar that can adapt to wide variations in edaphic and climate conditions. In this work, Paulownia Clone in Vitro 112 leaves were separated into an extract and four fractions (A–D) differing in chemical content in order to investigate their chemical content using LC-MS analysis. The extract and fractions were also evaluated for their anticoagulant and antioxidant properties in a human plasma in vitro. Results: The Paulownia leaf extract contained mainly phenolic compounds (e.g., verbascoside), small amounts of iridoids (e.g., aucubin or 7-hydroxytometoside) and triterpenoids (e.g., maslinic acid) were also detected. Our results indicate that the extract and fractions have different effects on oxidative stress in human plasma treated with H2O2/Fe in vitro, which could be attributed to differences in their chemical content. For example, the extract and all the fractions, at the two highest concentrations of 10 and 50 µg/mL, significantly inhibited the plasma lipid peroxidation induced by H2O2/Fe. Fractions C and D, at all tested concentrations (1–50 µg/mL) were also found to protect plasma proteins against H2O2/Fe-induced carbonylation. The positive effects of fraction C and D were dependent on the dose. Conclusions: The extract and all four fractions, but particularly fractions C and D, which are rich in phenolic compounds, are novel sources of antioxidants, with an inhibitory effect on oxidative stress in human plasma in vitro. Additionally, the antioxidant potential of fraction D may be associated with triterpenoids.

中文翻译：

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人血浆中泡桐克隆112叶提取物和四种成分的比较植物化学、抗氧化和止血研究

背景：泡桐克隆体外112，被称为oxytree或氧树，是泡桐和泡桐（泡桐科）的杂交克隆。oxytree 是一种快速生长的杂交品种，可以适应土壤和气候条件的广泛变化。在这项工作中，泡桐克隆体外 112 叶被分离为提取物和化学含量不同的四个部分 (A-D)，以便使用 LC-MS 分析研究它们的化学含量。还评估了提取物和级分在体外人血浆中的抗凝血和抗氧化特性。结果：泡桐叶提取物主要含有酚类化合物（如毛蕊花苷）、少量环烯醚萜类（如桃叶珊瑚苷或7-羟基甲壳素）和三萜类化合物（如山楂酸）。我们的结果表明，提取物和馏分对体外用 H2O2/Fe 处理的人血浆中的氧化应激有不同的影响，这可能是由于其化学成分的差异。例如，提取物和所有馏分，在 10 和 50 µg/mL 的两个最高浓度下，显着抑制了 H2O2/Fe 诱导的血浆脂质过氧化。还发现所有测试浓度 (1–50 µg/mL) 的组分 C 和 D 都能保护血浆蛋白免受 H2O2/Fe 诱导的羰基化。C 部分和 D 部分的积极作用取决于剂量。结论：提取物和所有四种组分，尤其是富含酚类化合物的组分 C 和 D，是抗氧化剂的新来源，对体外人血浆中的氧化应激具有抑制作用。此外，