ABSTRACT

To explore the association between polymorphisms in microRNAs (miRNAs) and the cholinesterase (ChE) activity in omethoate-exposed workers, we recruited 180 omethoate-exposed workers and 115 controls to measure their ChE activity using acetylcholine and dithio-bis-(nitrobenzoic acid) and genotype susceptible SNPs in their miRNA by time-of-flight mass spectrometry. ChE activity in the exposure group was lower than that in the control group (P < 0.001). The analysis of covariance result showed that ChE activity was lower in the (- -/- T) genotype in miR-30a rs111456995 (1.97 ± 0.47) than in the TT genotype (2.23 ± 0.59) of the exposure group (P = 0.004). Multivariate linear regression was performed to find influencing factors on ChE activity, and variables kept in the model included omethoate exposure (b = −1.094, P < 0.001), gender (b = −0.381, P < 0.001), miR-30a rs111456995 (- -/- T)(b = −0.248, P < 0.001), and drinking (b = 0.258, P =0.019). The results suggest that individuals carrying a (- -/- T) genotype in miR-30a rs111456995 were more susceptible to damage in their cholinesterase induced by omethoate exposure.

摘要

为了探索氧化乐果暴露工人中 microRNA (miRNA) 中的多态性与胆碱酯酶 (ChE) 活性之间的关联，我们招募了 180 名暴露于氧化乐果的工人和 115 名对照，以使用乙酰胆碱和二硫代双-(硝基苯甲酸) 测量他们的胆碱酯酶 (ChE) 活性通过飞行时间质谱法对 miRNA 中的易感 SNP 进行基因分型。暴露组ChE活性低于对照组（P <0.001）。协方差分析结果显示， miR-30a rs111456995（1.97±0.47） （--/-T）基因型ChE活性低于暴露组TT基因型（2.23±0.59）（P= 0.004)。进行多元线性回归以寻找影响ChE活性的因素，模型中保留的变量包括氧化乐果暴露（b = -1.094，P <0.001），性别（b = -0.381，P <0.001），miR-30a rs111456995（ - -/- T)( b = -0.248, P < 0.001) 和饮酒 ( b = 0.258, P = 0.019)。结果表明，在miR-30a rs111456995 中携带 (- -/- T) 基因型的个体更容易受到氧化乐果引起的胆碱酯酶损伤。