ABSTRACT

Because the small intestine is exposed to variety of foreign substances, it participates in host immune response. We investigated whether the expression levels of intestinal MAdCAM-1, PECAM-1 (CD31) and CAV-1 are affected by endoplasmic reticulum (ER) stress following brief treatment with tunicamycin (TN). We administered a single dose of TN intraperitoneally. Twenty-four hours later, MAdCAM-1, PECAM-1 and CAV-1 expression levels in Peyer’s patches and villi were examined using immunohistochemistry (IHC), immunofluorescence (IF) and western blotting. Immunostaining of MAdCAM-1 and CAV-1 in control and TN treated Peyer’s patches and villi exhibited similar staining patterns. The immunoreactivity of PECAM-1 was similar for the control and TN treated Payer’s patches, whereas staining was decreased significantly in TN treated villi. Our findings suggest that short term TN treatment did not affect leukocyte movement to lymphoid compartments of the small intestine, but it altered villus architecture due to decreased PECAM-1 expression.

摘要

由于小肠暴露于多种外来物质，因此参与宿主免疫反应。我们研究了用衣霉素 (TN) 短暂处理后肠道 MAdCAM-1、PECAM-1 (CD31) 和 CAV-1 的表达水平是否受到内质网 (ER) 应激的影响。我们腹膜内注射了单剂量的 TN。24 小时后，使用免疫组织化学 (IHC)、免疫荧光 (IF) 和蛋白质印迹法检查派耶氏斑和绒毛中 MAdCAM-1、PECAM-1 和 CAV-1 的表达水平。MAdCAM-1 和 CAV-1 的免疫染色在对照和 TN 处理的 Peyer 集结和绒毛中表现出相似的染色模式。PECAM-1 的免疫反应性对于对照和 TN 处理的 Payer's 斑块是相似的，而在 TN 处理的绒毛中染色显着降低。