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Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events
eLife ( IF 7.7 ) Pub Date : 2020-09-23 , DOI: 10.7554/elife.58771
Jun Kurushima 1 , Nathalie Campo 2 , Renske van Raaphorst 1 , Guillaume Cerckel 1 , Patrice Polard 2 , Jan-Willem Veening 1
Affiliation  

The spread of antimicrobial resistance and vaccine escape in the human pathogen Streptococcus pneumoniae can be largely attributed to competence-induced transformation. Here, we studied this process at the single-cell level. We show that within isogenic populations, all cells become naturally competent and bind exogenous DNA. We find that transformation is highly efficient and that the chromosomal location of the integration site or whether the transformed gene is encoded on the leading or lagging strand has limited influence on recombination efficiency. Indeed, we have observed multiple recombination events in single recipients in real-time. However, because of saturation and because a single-stranded donor DNA replaces the original allele, transformation efficiency has an upper threshold of approximately 50% of the population. The fixed mechanism of transformation results in a fail-safe strategy for the population as half of the population generally keeps an intact copy of the original genome.

中文翻译:

肺炎球菌转化过程中的无偏同源重组允许多个染色体整合事件

人类病原体肺炎链球菌中抗菌素耐药性的传播和疫苗逃逸在很大程度上可归因于能力诱导的转化。在这里,我们在单细胞水平上研究了这个过程。我们表明,在同基因群体中,所有细胞都具有自然能力并结合外源 DNA。我们发现转化效率很高,整合位点的染色体位置或转化的基因是否编码在前导链或滞后链上对重组效率的影响有限。事实上,我们已经实时观察到单个受体中的多个重组事件。然而,由于饱和以及单链供体 DNA 取代了原始等位基因,转化效率的上限约为种群的 50%。
更新日期:2020-09-23
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