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Overexpression of Arabidopsis microRNA167 induces salicylic acid‐dependent defense against Pseudomonas syringae through the regulation of its targets ARF6 and ARF8
Plant Direct ( IF 3 ) Pub Date : 2020-09-23 , DOI: 10.1002/pld3.270
Julie C. Caruana 1, 2 , Nikhilesh Dhar 1, 3 , Ramesh Raina 1
Affiliation  

microRNAs are powerful regulators of growth, development, and stress responses in plants. The Arabidopsis thaliana microRNA miR167 was previously found to regulate diverse processes including flower development, root development, and response to osmotic stress by controlling the patterns of expression of its target genes AUXIN RESPONSE FACTOR 6 (ARF6), ARF8, and IAA‐Ala RESISTANT 3. Here, we report that miR167 also modulates defense against pathogens through ARF6 and ARF8. miR167 is differentially expressed in response to the bacterial pathogen Pseudomonas syringae, and overexpression of miR167 confers very high levels of resistance. This resistance appears to be due to suppression of auxin responses and is partially dependent upon salicylic acid signaling, and also depends upon altered stomatal behavior in these plants. Closure of stomata upon the detection of P. syringae is an important aspect of the basal defense response, as it prevents bacterial cells from entering the leaf interior and causing infection. Plants overexpressing miR167 constitutively maintain small stomatal apertures, resulting in very high resistance when the pathogen is inoculated onto the leaf surface. Additionally, the systemic acquired resistance (SAR) response is severely compromised in plants overexpressing miR167, in agreement with previous work showing that the activation of SAR requires intact auxin signaling responses. This work highlights a new role for miR167, and also emphasizes the importance of hormonal balance in short‐ and long‐term defense and of stomata as an initial barrier to pathogen entry.

中文翻译:

拟南芥microRNA167的过表达通过调控靶标ARF6和ARF8诱导水杨酸对丁香假单胞菌的防御

microRNA是植物生长,发育和胁迫反应的有力调节剂。的拟南芥微小RNA miR167以前发现通过控制其靶基因的表达的模式来调节不同的过程,包括花发育,根发育,并响应于渗透胁迫6(ARF6),ARF8,生长素应答因子IAA丙氨酸RESISTANT 3。在这里,我们报道miR167还通过ARF6ARF8调节对病原体的防御。miR167响应细菌病原体假单胞菌假单胞菌的反应而差异表达。miR167赋予很高的抗性水平。这种抗性似乎是由于生长素应答的抑制,部分取决于水杨酸信号传导,也取决于这些植物中气孔行为的改变。检测到丁香假单胞菌后气孔的关闭是基础防御反应的重要方面,因为它可以防止细菌细胞进入叶片内部并引起感染。过度表达miR167的植物组成性地维持较小的气孔孔径,当病原体接种到叶片表面时会产生很高的抗性。此外,在过表达miR167的植物中,系统获得性抗性(SAR)反应受到严重损害与先前的研究表明SAR的激活需要完整的生长素信号转导反应相一致。这项工作强调了miR167的新作用,也强调了激素平衡在短期和长期防御中的重要性以及气孔作为病原体进入的初始障碍的重要性。
更新日期:2020-09-23
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